Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) ab175652 from Abcam

Product Specs
ItemGoat Anti-Rabbit IgG H&L (Alexa Fluor® 405)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab175652
Concentration2 mg/ml
ClonalityPolyclonal
TargetIgG
ApplicationsELISA, FCM, ICC, IF, IHC-fr, IHC-p
Antibody TypeSecondary
HostGoat
FormatThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads
Conjugate/TagAlexa Fluor® 405
Quantity500 UG

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Lactate promotes Salmonella intracellular replication and systemic infection via driving macrophage M2 polarizationMicrobiology SpectrumDecember 12, 2023Xinyue Wang, Bin Yang, Shuangshuang Ma, Xiaolin Yan, Shuai Ma, Hongmin Sun, Yuyang Sun, Lingyan Jiangincubated with fluorophore-conjugated secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor 405) (Abcam, ab175652; 1:500 dilution) at room temperature for 1 h. The stained cells were visualized using a Zeiss LSM800

(2) Leptospira Lipid A Is a Potent Adjuvant That Induces Sterilizing Immunity against LeptospirosisVaccines (Basel)December 6, 2023Vivek P. Varma, Mohammad Kadivella, Sridhar Kavela, Syed M. Faisaland then incubated with the secondary antibody, goat anti-rabbit Alexa Fluor® 405-conjugated IgG H&L (ab175652, Abcam, 1:300), for two hours at room temperature. The antibody-stained sectioned slides were mounted

(3) Identifying dysregulated immune cell subsets following volumetric muscle loss with pseudo-time trajectoriesCommunications BiologyJuly 19, 2023Lauren A. Hymel, Shannon E. Anderson, Thomas C. Turner, William Y. York, Hongmanlin Zhang, Adrian R. Liversage, Hong Seo Lim, Peng Qiu, Luke J. Mortensen, Young C. Jang, et al.prior to SHG imaging followed by staining with secondary antibody conjugated to Alexa Fluor 421 (Abcam, ab175652, 1:250) and Alexa Fluor 488-conjugated CD206 (BioLegend, 141710, 1:150). Slides prepared for SHG were

(4) Effect of Photobiomodulation on Protein Kinase Cδ, Cytochrome C, and Mitochondria in U87 MG CellsCellsMay 22, 2023Viktória Pevná, Georges Wagnières, Daniel Jancura, Veronika HuntošováAlexaFluor 546 (1:1000, A-11030, Thermo Fisher Scientific, Waltham, MA, USA), and AlexaFluor 405 (1:1000, ab175652, Abcam, Cambridge, UK), and diluted in 1% BSA, was applied to the cells for 1 h at room temperature.

(5) Parkin Precipitates on Mitochondria via Aggregation and AutoubiquitinationInternational journal of molecular sciencesMay 19, 2023Ardah MT, Radwan N, Khan E, Kitada T, Haque ME.anti-mouse Alexa Fluor (Invitrogen; 594 A-11032,) (1:500) and Goat anti-rabbit Alexa Fluor 405 (Abcam; ab175652) (1:200). A Fluoroshield (Sigma; F6182) was used for their mounting. Images were visualized and captured

(6) Single-cell analysis of peripheral blood from high-altitude pulmonary hypertension patients identifies a distinct monocyte phenotypeNature CommunicationsMarch 31, 2023Xin-Hua Wu, Yang-Yang He, Zhang-Rong Chen, Ze-Yuan He, Yi Yan, Yangzhige He, Guang-Ming Wang, Yu Dong, Ying Yang, Yi-Min Sun, et al.antibody [EP1176Y] (Abcam, #ab52917, 1:30 dilution) and Goat Anti-Rabbit IgG H&L -AF405/DAPI (Abcam, #ab175652, 1:2000 dilution) as the secondary antibody were used for labeling intracellular expression of HIF1α

(7) Delayed denervation-induced muscle atrophy in Opg knockout miceFrontiers in PhysiologyFebruary 22, 2023Mingming Zhang, Ming Chen, Yi Li, Man Rao, Duanyang Wang, Zhongqi Wang, Licheng Zhang, Pengbin Yin, Peifu Tangincubated for 1 h at room temperature with a secondary antibody solution containing Alexa Fluor® 405 (ab175652, Abcam), Alexa Fluor® 488 (ab150121, Abcam), Alexa Fluor™ 568 (A-21124, Invitrogen) and Alexa Fluor®

(8) Enhanced liquidity of p62 droplets mediated by Smurf1 links Nrf2 activation and autophagyCell & bioscienceFebruary 21, 2023Xia Q, Li Y, Xu W, Wu C, Zheng H, Liu L, Dong L.Alexa Fluor® 647 donkey anti-goat IgG (Abcam, ab150131), Alexa Fluor® 405 goat anti-rabbit IgG (Abcam, ab175652). Preparing mouse embryonic fibroblasts (MEFs) from mouse embryos Primary MEFs were obtained from

(9) Differentiation-dependent changes in lamin B1 dynamics and lamin B receptor localizationMolecular biology of the cellFebruary 1, 2023Wesley CC, Levy DL.(Active Motif; 39285), goat anti-rabbit IgG H&L Alexa Fluor 405 used at 1:200 for immunofluorescence (Abcam; ab175652), donkey anti-mouse IgG H+L Alexa Fluor 647 used at 1:1000 for immunofluorescence (ThermoFisher; A-31571),

(10) Tissue libraries enable rapid determination of conditions that preserve antibody labeling in cleared mouse and human tissueeLifeJanuary 19, 2023Zwang TJ, Bennett RE, Lysandrou M, Woost B, Zhang A, Lieber CM, Richardson DS, Hyman BT.(Santa Cruz Biotechnology, SC5303)Secondary antibody Goat Anti-Rabbit IgG H&L conjugated to Alexa Fluor 405 (Abcam, ab175652) and Alexa Fluor 647 (ab150079).Streptavidin conjugated to Alexa Fluor 568 (Thermo Fisher, S11226). Each

(11) Interchromosomal interaction of homologous Stat92E alleles regulates transcriptional switch during stem-cell differentiationNature CommunicationsDecember 1, 2022Matthew Antel, Romir Raj, Madona Y. G. Masoud, Ziwei Pan, Sheng Li, Barbara G. Mellone, Mayu Inabadilution of 1:400. Secondary antibodies used were Goat Anti-Rabbit IgG H&L (Alexa Fluor 488, Abcam, ab175652), Goat Anti-Rabbit IgG H&L (Alexa Fluor 647, Abcam, ab150079), Goat Rat IgG H&L (Alexa Fluor 488, Abcam,

(12) SARS-CoV-2 infects adipose tissue in a fat depot- and viral lineage-dependent mannerNature communicationsSeptember 29, 2022Saccon TD, Mousovich-Neto F, Ludwig RG, Carregari VC, Dos Anjos Souza AB, Dos Passos ASC, Martini MC, Barbosa PP, de Souza GF, Muraro SP, et al.Alexa Fluor 488 (Thermo Fisher Scientific, A21202, 1:800), 594 (Abcam, ab150116, 1:800), or 405 (Abcam, ab175652, 1:800). Nucleus staining was carried out using 4′,6-diamidino-2-phenylindole dihydrochloride dye (DAPI,

(13) In vitro reconstitution of calcium-dependent recruitment of the human ESCRT machinery in lysosomal membrane repairProceedings of the National Academy of Sciences of the United States of AmericaAugust 30, 2022Shukla S, Larsen KP, Ou C, Rose K, Hurley JH.using secondary-conjugated goat antibodies targeting the species of ALG-2 and ALIX antibodies (no. ab175652, ab150113, Abcam). Secondary antibodies were diluted at 1:500 with 2% BSA in PBS and incubated with

(14) Biomechanical properties of native and cultured red blood cells–Interplay of shape, structure and biomechanicsFrontiers in PhysiologyAugust 16, 2022Claudia Bernecker et al.Kit, respectively (all Abcam, 1:200). Secondary antibodies Alexa Fluor® 405 Goat Anti-Rabbit (IgG) (ab175652) and Alexa Fluor® 488 Goat Anti-Mouse (IgG) (ab150113) (both Abcam, 1:1,000) were diluted in 4% goat

(15) The latent dedifferentiation capacity of newt limb muscles is unleashed by a combination of metamorphosis and body growthScientific reportsAugust 1, 2022Yu ZY, Shiga S, Casco-Robles MM, Takeshima K, Maruo F, Chiba C.Thermo Fisher Scientific) and goat anti-rabbit IgG (H + L) antibody conjugated with Alexa Fluor 405 (1:500; ab175652; Abcam). Tissue sections on coverslips were rinsed thoroughly (PBS, 1% Triton X-100 in PBS and PBS;

(16) Crosstalk between dihydroceramides produced by Porphyromonas gingivalis and host lysosomal cathepsin B in the promotion of osteoclastogenesisJournal of cellular and molecular medicineMay 1, 2022Duarte C, Yamada C, Garcia C, Akkaoui J, Ho A, Nichols F, Movila A.Donkey Anti‐Goat IgG H&L (Abcam, Cat# ab150129) and Alexa Fluor® 405 Goat Anti‐Rabbit IgG H&L (Abcam, Cat# ab175652). The nuclei were counterstained using Draq5TM (Thermo Scientific, Cat#62254) staining solution, and

(17) Insulin potentiates the response to capsaicin in dorsal root ganglion neurons in vitro and muscle afferents ex vivo in normal healthy rodentsThe Journal of physiologyFebruary 1, 2022Hori A, Hotta N, Fukazawa A, Estrada JA, Katanosaka K, Mizumura K, Sato J, Ishizawa R, Kim HK, Iwamoto GA, et al.fluorescence-conjugated secondary antibodies (Alexa Fluor 405-conjugated goat anti-rabbit IgG [1:100, Cat#ab175652, RRID#AB_2687498, Abcam, Cambridge, MA, USA]; Cy3 goat anti-mouse IgG [1:50, Cat#115–165–146, RRID#AB_2338690,

(18) COG133 Attenuates the Early Brain Injury Induced by Blood-Brain Barrier Disruption in Experimental Subarachnoid HemorrhageJournal of Healthcare EngineeringJanuary 7, 2022Yongfa Zhang, Baocheng Gao, Jingsong Ouyang, Bai Tai, Shuai ZhouGoat Anti-Rabbit IgG (1:200, ab150079, Abcam), DyLight 405 AffiniPure Goat Anti-Rabbit IgG (1:200, ab175652, Abcam), and DyLight 488 AffiniPure Rabbit Anti-Goat IgG (1:200, A23230, Abbkine). 4′, 6–105 diamidino-2-phenylindole

(19) Autophagy and Apoptosis Induced in U87 MG Glioblastoma Cells by Hypericin-Mediated Photodynamic Therapy Can Be Photobiomodulated with 808 nm LightBiomedicinesNovember 17, 2021Viktoria Pevna, Georges Wagnières, Veronika Huntosovawith AlexaFluor 488 (1:1000, ab150077, Abcam, Cambridge, UK) for Giantin and AlexaFluor 405 (1:1000, ab175652, Abcam, Cambridge, UK) for PICK1 were diluted in 1% BSA and applied to the cells for 1 h at room temperature.

(20) A mechanism of cooling hot tumors: Lactate attenuates inflammation in dendritic cellsiScienceSeptember 24, 2021Kanemaru H, Mizukami Y, Kaneko A, Tagawa H, Kimura T, Kuriyama H, Sawamura S, Kajihara I, Makino K, Miyashita A, et al.Motif,2C5 rabbit anti-mouse SRF antibody,Abcam,ab53147 goat anti-rabbit IgG H&L (Alexa Fluor 405),Abcam,ab175652 APC anti-human CD20 Antibody,BioLegend,2H7 Alexa Fluor 594 anti-human MART-1 antibody,BioLegend,M2-7C10 APC

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5/9/2021Good Secondary Antibody Alexa Fluor® 405 Abhijit NirwaneOur Study role of Basement membrane in Blood-brain barrier maintenance and function.

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) from Abcam

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) from Abcam

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