Fig 1: ELKS and its associated proteins and/or homologs are not necessary for LAMP1?-RUSH post-Golgi tubule fusion. (A) TIRF imaging of LAMP1?-RUSH (gray) cells expressing heterologous HALO-ELKS (red) mostly localized to fusion sites (blue arrowheads). From Video 10. Scale bar: 10 µm. (B) Cell-surface ratio quantification (flow cytometry) of LAMP1?-RUSH at the plasma membrane after stable ELKS KO and 35 min of biotin exposure. (C) Immunoblot confirming loss of ELKS in a stable LAMP1?-RUSH ELKS KO clonal cell line. (D) Cell-surface ratio quantification (flow cytometry) of LAMP1?-RUSH at the plasma membrane after transient ELKS KO and 35 min of biotin exposure. (E) Cell-surface ratio quantification (flow cytometry) of LAMP1?-RUSH at the plasma membrane after transient KO of known ELKS interaction partners and 35 min of biotin exposure. This experiment was carried out in a stable clonal ELKS KO cell line. (F) Cell-surface ratio quantification (flow cytometry) of LAMP1?-RUSH at the plasma membrane after transient KO of EXOC1, ELKS, and combined. Error bar = SD of at least three independent experimental repeats. Two-tailed t test was performed on data in B and D, and Tukey’s multiple comparisons test (HSD, FWER = 0.05) was performed on data in F. *P = 0.05; ***P = 0.001.
Fig 2: The development of dendritic spines and synapses of four groups mice: (a) the density and area of dendritic spines in prefrontal cortex (PFC) was assessed by Golgi stanning. Scale bar = 10 µm; (b) the density of total, mushroom, stubby, and filopodia; (c) the spine area of mushroom, stubby, and filopodia; (d) the schematic diagram of expression levels in ELKS, PSD95, and p-synapsin, measured by Western blot; (e–g) the semiquantitative analysis of the expression of ELKS (e), PSD95 (f), and p-synapsin (g). WT, wild type; KO, knockout; SR, sleep restriction. All groups: n = 3 (three times repeated). Data are the means ± SEM. *p < .05, **p < .01, ***p < .001, ****p < .0001.
Supplier Page from Abcam for Anti-ELKS antibody [EPR13777]