Fig 1: AKR1C Family Is Highly Upregulated in STK11/KEAP1 Co-mutants(A) Heatmap of AKR1C expression in isogenic knockout clones from three cell lines. Expression shown is a Z score of the normalized TPM after removing the effect of cell line. Hierarchical clustering was performed using Manhattan distance and the Ward D method of clustering.(B) Boxplots show normalized TPM (after removing cell line effect) of each AKR1C family member in three isogenic clones with indicated genotype.(C) Immunoblots of protein expression of AKR1C family members in isogenic clones.(D) Immunohistochemical staining for AKR1C1 in two independent tumor microarrays containing duplicate patient tumor samples from 60 (TMA1) and 58 (TMA2) patients with lung adenocarcinoma and known STK11 and KEAP1 status. Blind scoring was performed by a pathologist and retrospectively correlated to genotype for each tumor. Damaged samples were not considered in the scoring.
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