Fig 1: Representative images of staining by immunoblotting and immunohistochemistry in the colon of pigs. a, immunoblotting of Toll-like receptor 4 (TLR4), Nucleotide-binding oligomerization domain protein 1 (NOD1), Nuclear factor-?B (NF-?B) p65 and G-protein coupled receptor 43 (GPR43) protein. b, immunohistochemical staining of mucin-4 (MUC-4) (magnification: × 100). Values are means ± SEM (n = 7). LPA: Low-protein diets supplemented with free amino acids. LPC: Low-protein diets supplemented with casein hydrolysate
Fig 2: Sevoflurane blocks the activation of the TLR4/MyD88/NF-?B signaling pathway. (A) The protein expressions of the NF-?B signaling pathway transcription factors including MyD88, nuclear p-p65, total p-65, total I?B-a and p-I?B-a were detected by western blotting in four groups of mice: The Sham, ALI, ALI + SEVO and ALI + SEVO + antagomir-27a-3p groups (n=6 mice/group). (B) The bands were semi-quantitatively analyzed using Quantity One software, and normalized to ß-actin, total p65 and total I?B-a density, respectively. Data are presented as the mean ± standard deviation of three independent experiments. *P<0.05 and **P<0.01 vs. Sham group; ##P<0.01, as indicated. TLF4, Toll-like receptor 4; SEVO, the sevoflurane treated group; ALI, acute lung injury; p-, phosphorylated.
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