Fig 1: RPS12 overexpression affects heavy polysome association of APAF-1 and XIAP mRNAs. (A) Semi-quantitative RT-PCR was used to measure the abundance of APAF-1, XIAP, GAPDH, and β-Actin mRNAs in polysome fractions of normoxic (21% O2) and hypoxic (1% O2) HEK293 cells overexpressing RPS12 (eS12) with the Myc-DDK-RPS12 ORF vector (red line) or control cells expressing the empty vector pMyc-N1 (blue line). The fraction of mRNA in each lane was calculated based on total band intensity of the transcript across the entire polysome gradient. Dashed line denotes the start of the polysome fractions. A representative image is shown. (B) Total levels of APAF-1 and XIAP mRNA were measured in normoxic and hypoxic HEK293 control cells and those overexpressing RPS12 using RT-qPCR. The ΔΔCt method was used, normalizing to reference gene RPL13A, and fold-change made relative to empty vector control. Data (n = 3), mean ± SEM. Two-tailed unpaired t-tests were performed.
Fig 2: Analysis of Plasma Cell Subpopulations By scRNA-seq And Flow Cytometry. (A) A UMAP plot showing the subpopulations of plasma cells after performing integration of control kidney and ccRCC plasma cells. (B) Violin plots showing the distribution of the two featured genes (RPS12 and IGHG4) in integrated plasma cells. (C) Heatmap showing the heterogeneity of the top 10 genes expressed in the two plasma cell subpopulations. (D) Split UMAP plots showing the comparison of plasma cell subpopulations between control kidneys and ccRCC biopsies. (E) Stacked bar plots showing the comparison of plasma cell subpopulations between control kidneys and ccRCC biopsies. (F) Flow cytometry analysis of plasma cell subpopulations in control kidneys and ccRCC biopsies. (G) Scatter plots showing the statistical analysis of the two plasma cell subpopulations in control kidneys and ccRCC biopsies. Each dot represents one readout. The data represent 3 independent experiments. (H) Volcano plots showing the distribution of DEGs (ccRCC vs. Control) in the two plasma cell subpopulations. **p<0.01.
Supplier Page from Abcam for Anti-RPS12 antibody [EPR10963]