Fig 1: EHMT1 interacts with LMNB1 domain and co-regulate a subset of genes AEHMT1 interacts with EHMT2, LMNB1, and HP1. Whole cell lysates from fetal HDFs were subjected to IP reaction using EHMT1 or LMNB1 antibody. Subsequently, Western blot was performed using the IPed material to detect EHMT1, EHMT2, LMNB1, and HP1. ASH2L did not show any interaction with EHMT1 and LMNB1 thus acted as a negative control.BEHMT1 interacts with LMNB1 and EHMT2 via SET domain. HEK293 cells were transfected with pEGFP-Ankyrin (pEGFPC1-ANK) or pEGFPC1-SET domains of EHMT1 to determine domain-specific association with LMNB1. Cell extracts were subjected to IP using GFP-antibody, and the bound complexes were then analyzed by immunoblotting using LMNB1, EHMT2, and GFP antibodies. HEK-293 whole cell extract represents 1% input. pEGFPC1 empty vector transfected HEK293 or untransfected HEK293 cells were used as control reactions. Arrows indicate specific band.CCoomassie-stained SDS–PAGE gel showing 6X His EHMT1-SET purified protein used for methyltransferase assays.D–FGene ontology (GO) analysis of EHMT1 and LMNB1 bound genes. Representative figure showing enriched GO terms for EHMT1 (D), LMNB1 (E), and EHMT1 and LMNB1 (F) co-bound genes. The length of the bar (y-axis) denotes total genes falling within GO term.GCircos plot showing genome wide peak density of EHMT1 (green) and LMNB1 (red).