Fig 1: Effects of CHB-II-F on protein expression of NPY, AgRP, POMC, CART, OX1R, Ob-R, and GHSR in the hypothalamus of the transplanted H22 hepatocellular carcinoma model mice receiving chemotherapy (x¯±s, n = 6). Statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001 compared with the model (saline) group. # p < 0.05, ## p < 0.01 compared with the 5-FU group, ? p < 0.05 compared with the YZXJC group.
Fig 2: Rbm8a regulates the generation and migration of PV+, SST+, and NPY+ interneurons.A Cell number of PV+ interneurons was decreased, and the distribution of PV+ interneurons was altered in the cortex of Nes-cre; Rbm8afl/+ mice. Scale bar = 50 µm. Quantification of the distribution of PV+ interneurons in the cortex. There are significantly altered PV+ interneurons in bins I, II, IV, and V compared to Rbm8afl/+ mice (**p < 0.01, ***p < 0.0001; t test, n = 3). B PV+ cells in Nes-cre; Rbm8afl/+ mice are significantly larger than in Rbm8afl/+ mice. Scale bar = 50 µm. Quantification of the size of PV+ interneurons (***p < 0.001; t test; d = 0.562; Cohens d). C Nes-cre; Rbm8afl/+ mice have altered distribution of SST+ interneurons in the cortex. Scale bar = 50 µm. Quantification of the distribution of SST+ interneurons in the cortex. There are significant changes of SST+ interneurons in bins I and III, compared to Rbm8afl/+ mice (*p < 0.05; t test, n = 3). D SST+ cells in Nes-cre; Rbm8afl/+ mice are significantly larger than in Rbm8afl/+ mice. Scale bar = 50 µm. Quantification of the size of SST+ interneurons in the cortex (***p < 0.001; t test; d = 0.359; Cohens d). E Nes-cre; Rbm8afl/+ mice showed altered distribution of NPY+ interneurons in the cortex. Scale bar = 50 µm. There are significantly fewer NPY+ interneurons in bins I and II, compared to Rbm8afl/+ mice (*p < 0.05, **p < 0.01; t test, n = 3). F NPY+ cells in Nes-cre; Rbm8afl/+ mice are significantly larger than in Rbm8afl/+ mice. Scale bar, 50 µm. Quantification of the size of NPY+ interneurons in the cortex (***p < 0.001; t test; d = 0.793; Cohens d).
Fig 3: Effects of CHB-II-F on protein expression of NPY, AgRP, POMC, CART, OX1R, Ob-R, and GHSR in the hypothalamus of the transplanted H22 hepatocellular carcinoma model mice receiving chemotherapy detected by immunohistochemistry (x¯ ± s, n = 6). (A) Immunohistochemical pictures of NPY, AgRP, POMC, CART, OX1R, Ob-R, and GHSR protein expression in hypothalamus (× 400, scale bar = 200 µm). (B) NPY IOD values in different groups. (C) AgRP IOD values in different groups. (D) POMC IOD values in different groups. (E) CART IOD values in different groups. (F) OX1R IOD values in different groups. (G) Ob-R IOD values in different groups. (H) GHSR IOD values in different groups. Statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001 compared with the model (saline) group. # p < 0.05, ## p < 0.01 compared with the 5-FU group. ? p < 0.05 compared with the YZXJC group.
Fig 4: Effects of CHB-II-F on the content of NPY, OX1R, Ghrelin, Leptin, GAS, MTL, PGE2, and EGF in serum, SOD, and MDA in the jejunum of the transplanted H22 hepatocellular carcinoma model mice receiving chemotherapy (x¯ ± s, n = 6–8). Statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001 compared with the model (saline) group. # p < 0.05, ## p < 0.01 compared with the 5-FU group.
Fig 5: Effects of CHB-II-F on mRNA expression of NPY, AgRP, POMC, CART, OX1R, Ob-R,., and GHSR in the hypothalamus of the transplanted H22 hepatocellular carcinoma model mice receiving chemotherapy (x¯ ± s, n = 6). (A) NPY mRNA expression in different groups. (B) CART mRNA expression in different groups. (C) GHSR mRNA expression in different groups. (D) Ob-R mRNA expression in different groups. (E) POMC mRNA expression in different groups. (F) OX1R mRNA expression in different groups. (G) AgRP mRNA expression in different groups. (H) Gel electrophotogram of target gene NPY, AgRP, POMC, CART, OX1R, Ob-R, GHSR, and ß-actin amplification products of real-time fluorescent quantitation polymerase chain reaction (RTFQ-PCR). 1: AgRP; 2: NPY; 3: POMC; 4: CART; 5: OX1R; 6: Ob-R; 7: GHSR. (I) Gel electrophotogram of total RNA of tumor tissue. Randomly select three samples from each group and verify their integrity. 1–3: saline-treated controls (model); 4–6: 5-FU treated controls (0.02 g/kg); 7–9: YZXJC (0.78 g/kg); 10–12: CHB-II-F(H) (65 g/kg); 13–15: CHB-II-F(M) (32.5 g/kg); 16–18: CHB-II-F(L) (16.25 g/kg). Statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001 compared with the model (saline) group. # p < 0.05, ## p < 0.01 compared with the 5-FU group. ? p < 0.05 compared with the YZXJC group.
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