Fig 1: LINC00665 exercises its biological functions by mediating RAP1B. (a, b) CCK8 (a) and EdU assays (b) showed the regulation of LINC00665 and RAP1B on proliferation of OS cells. (c) Wound-healing assays showed the influence of LINC00665 and RAP1B on migration of OS cells; magnification, 40×. (d) Transwell assays displayed the influence of LINC00665 and RAP1B on invasion of OS cells, magnification, 100×. ∗P < 0.05.
Fig 2: LINC00665 upregulates the RAP1B expression via miR-708 and miR-142-5p. (a) Predicted binding sequences of miR-708 or miR-142-5p with 3′UTR of RAP1B mRNA. (b) Predicted binding sequences of miR-708 or miR-142-5p with 3′UTR of RAP1B mRNA were validated by luciferase reporter assays. (c) The efficiency of the overexpression and knockdown of miR-708 and miR-142-5p in OS cells detected by qRT-PCR. (d) Western blot assays indicated the impact of miR-708 and miR-142-5p on the protein level of RAP1B, as well as the impact of LINC00665 together with miR-708 or miR-142-5p on the protein level of RAP1B. (e) Expressions of RAP1B mRNA in paired OS and nontumorous normal tissues were detected by qRT-PCR. (f) Expressions of RAP1B mRNA in OS cell lines and normal osteoblast cell line were detected by qRT-PCR.
Supplier Page from Abcam for Anti-RAP1B antibody