Fig 1: MiR-7-5p mimic reversed the effect of LINC01272 on miR-7-5p and CRLS1 expression of LC cells.(A and B) Relative miR-7-5p expression between of A549 (A) and PC-9 (B) cells was detected through qRT-PCR after transfection of miR-7-5p mimic and LINC01272 overexpression plasmid. U6 was a loading control. (C and D) Relative CRLS1 mRNA expression between of A549 (C) and PC-9 (D) cells was tested by qRT-PCR after transfection of miR-7-5p mimic and LINC01272 overexpression plasmid. GAPDH was a loading control. (E and F) Relative CRLS1 protein expression between of A549 (E) and PC-9 (F) cells was assessed through western blot after transfection of miR-7-5p mimic and LINC01272 overexpression plasmid. GAPDH was a loading control. *p < 0.05, **p < 0.01, ***p < 0.001 vs. NC+MC group; ##p < 0.01, ###p < 0.001 vs. LINC01272+MC group; &p < 0.05, &&&p < 0.001 vs. NC+M group. All experiments were repeated independently at least three times. Data were performed as the means ± SD. Abbreviations: CRLS1, cardiolipin synthase 1; LC, lung cancer; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NC, negative control for LINC01272 overexpression plasmid; MC, miR-7-5p mimic control; M, miR-7-5p mimic.
Fig 2: CRLS1 functioned as a target of miR-7-5p.(A) The number of potential target genes for miR-7-5p from StarBase, miRDB, TargetScan, miRWalk and miRTarbase and TCGA-LUAD database. (B) The Venn diagram of potential target genes for miR-7-5p. (C) The binding sites between miR-7-5p and CRLS1. (D and E) Relative luciferase activity of A549 (D) and PC-9 (E) cells was assessed through dual-luciferase reporter analysis after transfection of CRLS1-WT and miR-7-5p mimic. (F) Relative miR-7-5p expression between LC and adjacent normal tissues was evaluated by qRT-PCR. (G) Relative CRLS1 expression between LC and adjacent normal tissues was measured through qRT-PCR. ***p < 0.001 vs. MC group. All experiments were repeated independently at least three times. Data were performed as the means ± SD. Abbreviations: CRLS1, cardiolipin synthase 1; TCGA-LUAD, The Cancer Genome Atlas Lung Adenocarcinoma; CRLS1-WT, wild-type CRLS1; LC, lung cancer; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; MC, miR-7-5p mimic control.
Supplier Page from Abcam for Anti-CRLS1 antibody