Anti-N6-methyladenosine (m6A) antibody ab151230 from Abcam

Product Specs
ItemAnti-N6-methyladenosine (m6A) antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab151230
Quantity100 UG
Conjugate/TagUnconjugated
IsotypeIgG
HostRabbit
Antibody TypePrimary
ClonalityPolyclonal
Concentration1 mg/ml
ApplicationsNucleotide Array
ReactivityDrosophila melanogaster, Human
TargetN6-methyladenosine (m6A)

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(1) N6-methyladenosine-regulated ADIRF impairs lung adenocarcinoma metastasis and serves as a potential prognostic biomarkerCancer Biology & TherapyDecember 31, 2023Yin Teng, Xiaohan Zhao, Yang Xi, Ninghua Fupurified using DNase (Sigma) and fragmented into ~ 100 nucleotides. m6A primary antibody (10 μg, Abcam, ab151230) linked to Magna ChIP protein/G magnetic beads was co-immunoprecipitated with the fragmented mRNA for

(2) A1BG-AS1 promotes adriamycin resistance of breast cancer by recruiting IGF2BP2 to upregulate ABCB1 in an m6A-dependent mannerScientific ReportsNovember 25, 2023Jian Wang, Jie Xu, Jie Zheng10 mM Tris–HCl, pH 7.5, 0.1% NP-40) and incubated with magnetic beads coated with IgG and anti-m6A (ab151230, Abcam, USA) antibodies for 1 h in order for binding. Next, the purified mRNAs and the complex was added

(3) YTHDF1 mitigates acute kidney injury via safeguarding m6A-methylated mRNAs in stress granules of renal tubulesRedox BiologyNovember 1, 2023Wenwen Yang, Mingchao Zhang, Jiacheng Li, Shuang Qu, Fenglian Zhou, Minghui Liu, Limin Li, Zhihong Liu, Ke Zenand anti-YTHDF3 (25537-1-AP, 1:100) were from Proteintech; anti-G3BP1 (ab56574, 1:200) and anti-m6A (ab151230, 1:100) were from Abcam. Secondary antibodies were all from Invitrogen (A-21206, A-21203, 1:1000). All

(4) Targeting histone deacetylase suppresses tumor growth through eliciting METTL14-modified m(6) A RNA methylation in ocular melanomaCancer communications (London, England)November 1, 2023Zhuang A, Gu X, Ge T, Wang S, Ge S, Chai P, Jia R, Fan X.was randomly fragmented using RNA fragmentation buffer and subjected to IP with an anti‐m6A antibody (ab151230, Abcam) and PGM magnetic beads in IP buffer supplemented with RNase inhibitor. The m6A‐containing RNA

(5) YTHDF2 promotes gallbladder cancer progression and gemcitabine resistance via m6A‐dependent DAPK3 degradationCancer ScienceNovember 1, 2023Xuesong Bai, Jiemin Chen, Wenqin Zhang, Shengnan Zhou, Liangbo Dong, Jianhao Huang, Xiaodong Hetotal of 100 μL precleaned Protein G Dynabeads (10003D; ThermoFisher) was mixed with 5 μg anti‐m6A Ab (ab151230; Abcam) or IgG isotype control Ab (#3900S; CST) overnight at 4°C with rotation. The total RNA mixed

(6) IGF2BP2 acts as a m6A modification regulator in laryngeal squamous cell carcinoma through facilitating CDK6 mRNA stabilizationCell Death DiscoveryOctober 10, 2023Xiaojun Tang, Qinglai Tang, Shisheng Li, Mengmeng Li, Tao Yangcells (Novocyte, Agilent, USA). Methylated m6A RNA immunoprecipitation (Me-RIP) Anti-m6A antibody (ab151230, Abcam, Cambridge, MA, USA) was employed to perform the Me-RIP as previously described [39]. qRT-PCR

(7) KIAA1429/VIRMA promotes breast cancer progression by m6 A-dependent cytosolic HAS2 stabilizationEMBO reportsOctober 9, 2023Na Li, Zhouting Zhu, Yufei Deng, Rachel Tang, Hui Hui, Yuqi Kang, Tariq M Ranafragmented mRNA was used for IP and incubated with 10 μl of 1 μg/μl rabbit anti‐m6A polyclonal Ab (ab151230, Abcam) in IP‐binding buffer (10 mM Tris–HCl, 150 mM NaCl, 0.1% NP‐40, pH 7.4) for 2 h at 4°C. Protein

(8) HNRNPC promotes collagen fiber alignment and immune evasion in breast cancer via activation of the VIRMA-mediated TFAP2A/DDR1 axisMol MedAugust 1, 2023Bin Lian, Shuxun Yan, Jiayi Li, Zhengyang Bai, Jinping LiIsolation Systems (61,006, ThermoFisher, USA). For experimental grouping, anti-m6A antibody (1:500, ab151230, Abcam, Cambridge, UK) or anti-IgG antibody (ab109489, 1:100, Abcam, Cambridge, UK) were added to IP

(9) Deubiquitinase USP9X stabilizes RNA m(6)A demethylase ALKBH5 and promotes acute myeloid leukemia cell survivalThe Journal of biological chemistryAugust 1, 2023Wang P, Wang J, Yao S, Cui M, Cheng Y, Liu W, Gao Z, Hu J, Zhang J, Zhang H.no.: sc-8017), K48-linkage specific polyubiquitin (CST; catalog no.: 8081), m6A (Abcam; catalog no.: ab151230), FLAG (Sigma; catalog no.: F2922), HA (Sigma; catalog no.: B9183), GFP (Proteintech; catalog no.: 66002-1-Ig),

(10) Epigenetically silenced lncRNA SNAI3-AS1 promotes ferroptosis in glioma via perturbing the m(6)A-dependent recognition of Nrf2 mRNA mediated by SND1Journal of experimental & clinical cancer research : CRMay 19, 2023Zheng J, Zhang Q, Zhao Z, Qiu Y, Zhou Y, Wu Z, Jiang C, Wang X, Jiang X.Biodyne Nylon Transfer Membranes (Pall) and cross-linked for 30 s by UVP (1.2 kJ). The anti-m6A antibody (ab151230, Abcam) was applied to determine the global m6A level. Methylated RNA immunoprecipitation (MeRIP) assay

(11) A role for N6-methyldeoxyadenosine in C. elegans mitochondrial genome regulationbioRxivMay 17, 2023Lantana K. Grub, James P. Held, Tyler J. Hansen, Samantha H. Schaffner, Marleigh R. Canter, Evi M. Malagise, Maulik R. Patelwith 5% milk. After blocking, the membrane was incubated with the primary antibody, anti-6mA (Abcam, ab151230), at 1:1000 in TBST with 5% milk overnight at 4 C. The membrane was then washed 3 times with TBST for

(12) RNA demethylase ALKBH5 promotes colorectal cancer progression by posttranscriptional activation of RAB5A in an m6A-YTHDF2-dependent mannerClinical and translational medicineMay 1, 2023Shen D, Lin J, Xie Y, Zhuang Z, Xu G, Peng S, Tang G, Bai L, Zhu M, Zhang Y, et al.dot‐blot assays as introduced by previous publication. 28 The primary antibody used was anti‐m6A (Abcam, ab151230). 2.10 RNA sequencing, methylated RNA immunoprecipitation and m6A sequencing (MeRIP‐seq), and data

(13) Upregulation of UBR1 m6A Methylation by METTL14 Inhibits Autophagy in Spinal Cord InjuryeNeuroApril 24, 2023Changsheng Wang, Xitian Zhu, Rongsheng Chen, Xiaobo Zhang, Nancheng Lianpurified by PolyATtract mRNA Isolation Systems (A-Z5300, A&D. Technology Corporation). Anti-m6A (1:500, ab151230, Abcam) or anti-IgG (1:100, Abcam) antibodies were added to the immunoprecipitation buffer (20 mm Tris,

(14) Intestinal transit amplifying cells require METTL3 for growth factor signaling, KRAS expression, and cell survivalbioRxiv : the preprint server for biologyApril 21, 2023Danan CH, Naughton KE, Hayer KE, Vellappan S, McMillan EA, Zhou Y, Matsuda R, Nettleford SK, Katada K, Parham LR, et al.m6A-enriched RNA was immunoprecipitated a second time using 2 µg of a second anti-m6A antibody (Abcam ab151230). Final cDNA Libraries were prepared from twice immunoprecipitated RNA and fragmented input total RNA

(15) Nuclear m6A reader YTHDC1 promotes muscle stem cell activation/proliferation by regulating mRNA splicing and nuclear exporteLifeMarch 9, 2023Yulong Qiao, Qiang Sun, Xiaona Chen, Liangqiang He, Di Wang, Ruibao Su, Yuanchao Xue, Hao Sun, Huating WangTechnologies) was washed three times with 1 ml IP buffer and incubated with 2 μg m6A antibody (Abcam, ab151230) at RT for 30 min. The conjugated Dynabeads-m6A-antibody was washed in 1 ml IP buffer three times and

(16) METTL3 regulates breast cancer-associated alternative splicing switchesOncogeneMarch 1, 2023Achour C, Bhattarai DP, Groza P, Román ÁC, Aguilo F.buffer (250 mM Tris pH 7.4, 500 mM NaCl, 0.25% NP-40) and incubated with 10 µg of m6A antibody (Abcam, ab151230) in the presence of RNase inhibitors, for 3 h at 4 °C. 30 µl of prewashed Surebeads Protein A magnetic beads

(17) Evaluation of N 6-methyldeoxyadenosine antibody-based genomic profiling in eukaryotesGenome ResearchMarch 1, 2023Brian M. Debo, Benjamin J. Mallory, Andrew B. Stergachis111–RRID:AB_2619891), referred hereafter as “SS2”;Rabbit polyclonal anti-N6-methyladenosine antibody (Abcam ab151230–RRID:AB_2753144), referred hereafter as “AB1”;Anti-N6-methyladenosine (m6A) antibody, rabbit monoclonal

(18) Identification of miR-30c-5p as a tumor suppressor by targeting the m(6) A reader HNRNPA2B1 in ovarian cancerCancer medicineFebruary 1, 2023Wu Q, Li G, Gong L, Cai J, Chen L, Xu X, Liu X, Zhao J, Zeng Y, Gao R, et al.with 10% goat serum. The sections were incubated with primary antibodies against m6A (1:200, Abcam, ab151230) at 4°C overnight and cultured with the biotin‐conjugated IgG the next day. The sections were then stained

(19) PCIF1-mediated deposition of 5'-cap N(6),2'-O-dimethyladenosine in ACE2 and TMPRSS2 mRNA regulates susceptibility to SARS-CoV-2 infectionProceedings of the National Academy of Sciences of the United States of AmericaJanuary 31, 2023Wang L, Wang S, Wu L, Li W, Bray W, Clark AE, Gonzalez GM, Wang Y, Carlin AF, Rana TM.material, and the remaining 90% was subjected to immunoprecipitation with anti-m6A antibody (Abcam, ab151230). Immunoprecipitated RNA was eluted with RLT buffer (QIAGEN, 160051456), purified by ethanol precipitation,

(20) New insights on the interaction between m6A modification and non-coding RNA in cervical squamous cell carcinomaWorld Journal of Surgical OncologyJanuary 30, 2023Guqun Shen, Fen Li, Yan Wang, Yongmei Huang, Gulibiya Aizezi, Jinrui Yuan, Cailing Ma, Chen Lintotal RNA was extracted from C33A cells by TRIzol (Invitrogen, CA, USA). One microgram of m6A antibody (ab151230; Abcam) was coupled to the mixture of Protein A beads (Thermo Fisher Scientific, Waltham, MA). Later,

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Anti-N6-methyladenosine (m6A) antibody from Abcam

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Anti-N6-methyladenosine (m6A) antibody from Abcam

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