Fig 2: Western blotting analysis showing protein expression of TXNRD1, HSPA4L and ATP1B1 in (A) the fresh pre-freeze (PF) and (B) frozen-thawed (FT) sperm from boars of the good and poor semen freezability (GSF and PSF, respectively) groups.

Fig 3: Relative mRNA expression of (A) TXNRD1, (B) HSPA4L and (C) ATP1B1 in fresh pre-freeze (PF) and frozen-thawed (FT) sperm from boars of the good and poor semen freezability (GSF and PSF, respectively) groups. Values are presented as the mean ± SEM. Expression values of TXNRD1, HSPA4L and ATP1B1 were normalized against the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) value of the control. Significant at * p < 0.05, and ** p < 0.01 after the Bonferroni correction.

Fig 4: Relative expression of (A) TXNRD1, (B) HSPA4L and (C) ATP1B1 proteins in the fresh pre-freeze (PF) and frozen-thawed (FT) sperm from boars of the good and poor semen freezability (GSF and PSF, respectively) groups. Values represent the mean ± SEM. ß-actin was used as the control to normalize the relative expression of the analyzed proteins. Significant at * p < 0.05, and ** p < 0.01 after the Bonferroni correction.