Fig 1: Immunocompetent cervical epithelium trial. Haematoxylin and eosin (H&E) stained micrographs (200× magnification, cropped) and immunofluorescence micrographs (400× magnification) of five cervical organoids with differential purity and ratios of Langerhans cells to keratinocytes. Nuclei are stained blue (via DAPI), green represents pan-cytokeratin immunostaining (CKs, a cocktail of cytokeratin 14 and 19, rabbit monoclonals anti-human cytokeratin 14, clone EPR17350, Abcam, Cat. #ab181595 and cytokeratin 19, clone EP1580Y, Abcam, Cat. #ab52625 with secondary antibody AlexaFluor 488 donkey anti-rabbit, Invitrogen, Cat. #A21206), and red represents langerin (CD207, mouse monoclonal anti-human langerin/CD207, clone 310F7.02, Novus Biologicals, Cat. #DDX0361P-100 and secondary antibody AlexaFluor 594 donkey anti-mouse, Invitrogen, Cat. #A21203). Using formalin-fixed and paraffin-embedded tissue, antigen retrieval had to be performed with Tris-HCl (pH 9) rather than citrate buffer (pH 6). Only then, could langerin+ (CD207) LCs be detected at a physiologically relevant level (~1%) in rafts of group 3. Groups 4 and 5 resulted in an LC detection rate of >1%, but at the cost of epithelial thickness. It should be noted that the langerin+ micrographs presented in this figure are not a complete reflection of the ratio that has identified in the organoids.