Fig 1: Effect of BC-1215 on Fbxl2, Fbxo3, and TRAF1–6 protein expressions in lung tissue. Western blot and densitometry analysis of TRAF1 (A), TRAF2 (B), TRAF3 (C), TRAF4 (D), TRAF5 (E), TRAF6 (F), Fbxo3 (G), and Fbxl2 (H) protein in the lung tissue. ß-actin served as loading control for cytoplasmic proteins. Representative blots are shown. Ischemia-reperfusion (I/R) significantly increased protein expression of TRAF1–6 and Fbxo3 and decreased Fbxl2 protein expression in lung tissue compared with the control group. BC-1215 significantly decreased the degree of TRAF1-6 and Fbxo3 protein expression and increased Fbxl2 protein expression in the I/R group. Data are expressed as mean ± SD (n = 3 per group). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group; +p < 0.05, ++p < 0.01, +++p < 0.001 compared with the I/R group.