Fig 2: Silencing Stat1 promotes the expression of antioxidant genes in high glucose injured MPC-5 cells. a The Catalase, MnSOD, NQO1 and HO1 protein levels were detected by western blot in MPC-5 cells. b Relative protein expression of Catalase showed as bar diagrams. c Relative protein expression of MnSOD showed as bar diagrams. d Relative protein expression of NQO1 showed as bar diagrams. e Relative protein expression of HO1 showed as bar diagrams. f mRNA expression of Catalase was assessed using qRT-PCR in MPC-5 cells. g mRNA expression of MnSOD was assessed using qRT-PCR in MPC-5 cells. h mRNA expression of NQO1 was assessed using qRT-PCR in MPC-5 cells. i mRNA expression of HO1 was assessed using qRT-PCR in MPC-5 cells. GAPDH served as an internal control. Data were expressed as mean ± SD from three independent experiments. (*Compared with control, #Compared with HG, */#P < 0.05, **P < 0.01)

Fig 3: The effect of simultaneous silencing Stat1 and FoxO1 on the expression of antioxidant genes, Stat1 and FoxO1. The experiments divided were into six groups, which were control, HG (30 μM), siStat1 + HG (Stat1 siRNA + 30 μM HG), siStat1 + siFoxO1 + HG (Stat1 siRNA + FoxO1 siRNA + 30 μM HG), siFoxO1 + HG (FoxO1 siRNA + 30 μM HG) and siFoxO1 (FoxO1 siRNA). a The Catalase, MnSOD, NQO1, HO1, Stat1 and FoxO1 protein levels were detected by western blot in MPC-5 cells. b Relative protein expression of Catalase showed as bar diagrams. c Relative protein expression of MnSOD showed as bar diagrams. d Relative protein expression of NQO1 showed as bar diagrams. e Relative protein expression of HO1 showed as bar diagrams. f Relative protein expression of Stat1 showed as bar diagrams. g Relative protein expression of FoxO1 showed as bar diagrams. GAPDH served as an internal control. Data were expressed as mean ± SD from three independent experiments. (*Compared with control, # Compared with siStat1 + HG, */#P < 0.05, **/##P < 0.01)