Fig 2: Expression of ACE2 and TMPRSS2 in exocrine pancreatic cells. (A) iPanEXO Ductal cells CK19 (red) exhibiting i. ACE2 (green) and ii. TMPRSS2 (green) expression. (B) iPanEXO Acinar cells Amylase (red), MIST1 (gray) exhibit i. ACE2 (green) expression and ii. iPanEXO Acinar CTRC (red) exhibit TMPRSS2 (green). (C) iPanEXO Acinar and iPanENDO cultures contain some endocrine C-peptide expressing cells, which also co-stain with ACE2. (D) iPSC-derived pancreatic exocrine cells as well as human acinar tissues and human ductal cell line H6C7 express ACE2 and TMPRSS2. Data is shown as mean ± SEM with statistical significance determined by unpaired two-tailed t-test. **p < 0.01 and ****p < 0.0001. Scale bar represents 100 µm, and 20 µm for zoomed panels adjacent to main images. ICC images shown here are representative results from 27 independent sites acquired. RNA was extracted from aggregates of 2-3 biological replicates (using 12 well-plates), and qPCR was run with 3 technical replicates per sample. These results were pooled from 2 independent rounds of infection experiments.