FITC anti-human SSEA-4 330409 from BioLegend

Product Specs
ItemFITC anti-human SSEA-4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number330409
IsotypeIgG3, Kappa
CloneMC-813-70
Quantity25 tests
ReactivityHuman
ApplicationsFlow Cytometry (FC)
Conjugate/TagFITC
HostMouse
Antibody TypePrimary
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA); may contain stabilizer
ImmunogenHuman embryonic carcinoma cell line 2102Ep
ClonalityMonoclonal
TargetSSEA-4

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Website: biolegend.com

(1) Feeder cells treated with ethanol can be used to maintain self-renewal and pluripotency of human pluripotent stem cellsFEBS open bioFebruary 1, 2023Ren Y, Zhang S, Liang Y, Gong Z, Cui Y, Song W.Eppendorf tubes and incubated in 100 μL staining buffer supplemented with PE‐anti‐SSEA‐4 antibody (#330409; Biolegend, Beijing, China) for 30 min on ice, protected from light. Cells were washed twice and resuspended

(2) Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem CellsCell transplantationJanuary 1, 2023Lin CY, Ching YY, Wu SF, Lee YK, Fan HC, Su LY, Tsai SY, Chen YC, Shen CI, Su HL.against stage-specific embryonic antigen 4 (SSEA4; 1:100, fluorescein isothiocyanate [FITC]-conjugated, 330410, Biolegend) and Tra-1-60 (1:50, Alexa Fluor 647-conjugated, 560850, Beckton-Dickinson, BD, Franklin

(3) Human iPS cells engender corneal epithelial stem cells with holoclone-forming capabilitiesiScienceJune 25, 2021Watanabe S, Hayashi R, Sasamoto Y, Tsujikawa M, Ksander BR, Frank MH, Quantock AJ, Frank NY, Nishida K.conjugate,Life Technologies,Cat#A-11055; RRID:AB_2534102 Mouse anti-SSEA4 FITC conjugate,BioLegend,Cat#330410; RRID:AB_1089204 Mouse anti-CD104 PE conjugate,BioLegend,Cat#327808; RRID:AB_2129146 Mouse anti-CD200

(4) Transplantation of human amniotic epithelial cells promotes morphological and functional regeneration in a rat uterine scar modelStem cell research & therapyMarch 24, 2021Fan Y, Sun J, Zhang Q, Lai D.antibodies (CD146-PE: 361006, isotype control 400114; CD324-APC: 324108, isotype control 400122; SSEA4-FITC: 330410, isotype control 401317; HLADR-FITC: 11-9956-42; isotype control 11-4724-82; Biolegend, USA) at 4 °C

(5) Mesenchymal stem cells promote chemoresistance by activating autophagy in intrahepatic cholangiocarcinomaOncology reportsJanuary 1, 2021Gan L, Shen H, Li X, Han Z, Jing Y, Yang X, Wu M, Xia Y.obtain single-cell suspension. The suspension was then incubated with FITC-conjugated SSEA-4 (cat. no. 330409; BioLegend, lnc.) for 30 min at 4°C. Cells were washed 3 times with PBS, and then FITC-positive cells

(6) Direct Conversion of Human Stem Cell-Derived Glial Progenitor Cells into GABAergic InterneuronsCellsNovember 10, 2020Giacomoni J, Bruzelius A, Stamouli CA, Rylander Ottosson D.cat. no. 130-113-668, Bergisch Gladbach, Germany]; FITC anti-human SSEA-4, 1:20 [Biolegend, cat. no. 330410, San Diego, CA, USA] was performed for 15 min at 4 °C. Cells were washed once using Miltenyi wash buffer,

(7) Human induced pluripotent stem cell line banking for the production of rare blood type erythrocytesJournal of translational medicineJune 12, 2020Park YJ, Jeon SH, Kim HK, Suh EJ, Choi SJ, Kim S, Kim HO."BD Biosciences, Cat# 560193" Embryonic stem cell markers,FITC mouse anti-human SSEA4,"BioLegend, Cat# 330410" Differentiation markers,FITC mouse anti-human CD34,"BD Biosciences, Cat# 555821" Differentiation

(8) Identification of ALPPL2 as a Naive Pluripotent State-Specific Surface Protein Essential for Human Naive Pluripotency RegulationCell reportsMarch 17, 2020Bi Y, Tu Z, Zhang Y, Yang P, Guo M, Zhu X, Zhao C, Zhou J, Wang H, Wang Y, et al.

(9) Human amniotic epithelial cells improve fertility in an intrauterine adhesion mouse modelStem cell research & therapyAugust 14, 2019Li B, Zhang Q, Sun J, Lai D.hAEC characterization, cells were harvested and incubated with labeled primary antibodies (SSEA4-FITC: 330410, isotype control 401317; CD324-APC: 324108, isotype control 400122; CD146-PE: 361006, isotype control

(10) Methanol fixed fibroblasts serve as feeder cells to maintain stem cells in the pluripotent state in vitroScientific reportsMay 17, 2018Ren Y, Ma Z, Yu T, Ling M, Wang H.buffer supplemented with FITC-anti-SSEA-1 (#560886, BD pharmingem, USA) or PE-anti-SSEA-4 antibody (#330409, Biolegend, USA) for 30 min on ice, protected from light. Cells were washed twice and resuspended in

(11) ASXL3 Is a Novel Pluripotency Factor in Human Respiratory Epithelial Cells and a Potential Therapeutic Target in Small Cell Lung CancerCancer researchNovember 15, 2017Shukla V, Rao M, Zhang H, Beers J, Wangsa D, Wangsa D, Buishand FO, Wang Y, Yu Z, Stevenson HS, et al.

(12) Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cellsNature protocolsApril 1, 2017Hayashi R, Ishikawa Y, Katori R, Sasamoto Y, Taniwaki Y, Takayanagi H, Tsujikawa M, Sekiguchi K, Quantock AJ, Nishida K.isotype control IgG (MOPC-173; BioLegend, #400214) 11 FITC-conjugated SSEA-4 (MC-813-70; BioLegend, #330410) 12 FITC-conjugated isotype control IgG (MG3-35; BioLegend, #401317) 13 Alexa647-conjugated TRA-1-60

(13) Fabrication of a Corneal Epithelial Cell Sheet from Human Pluripotent Stem Cells by a Method Based on Spontaneous Ocular Cell DifferentiationProtocol ExchangeFebruary 18, 2016Ryuhei Hayashi, Ryuhei Hayashi, Yuki Ishikawa, Ryosuke Katori, Yuki Taniwaki, Motokazu Tsujikawa, Andrew J. Quantock, Kohji Nishida#HPS0063) PE-conjugated CD104 \(58BX4; Bio Legend, #327808) FITC-conjugated SSEA-4 \(MC813-70; BioLegend, #330410) Alexa647-conjugated TRA-1-60 \ (TRA-1-60-R; BioLegend, #330606) Anti-PAX6 antibody \(PRB-278P; BioLegend,

(14) Functional maintenance of differentiated embryoid bodies in microfluidic systems: a platform for personalized medicineStem cells translational medicineMarch 1, 2015Guven S, Lindsey JS, Poudel I, Chinthala S, Nickerson MD, Gerami-Naini B, Gurkan UA, Anchan RM, Demirci U.

(15) Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditionsNature protocolsNovember 1, 2012Beers J, Gulbranson DR, George N, Siniscalchi LI, Jones J, Thomson JA, Chen G.peak. Antibodies: OCT4-Alexa-488 (Millipore, cat. no. FCMAB113A4); SSEA4-FITC (Biolegend, cat. no. 330410); Tra1-60-FITC (Millipore, cat. no. FCMAB115F); msIGg-FITC and nonimmune control (Millipore, cat. no.

(16) Circular dichroism studies on native and phenylmethanesulfonyl-mesentericopeptidase. Denaturation in the presence of ureaInternational journal of peptide and protein researchFebruary 1, 1978N Genov, M Shopova

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FITC anti-human SSEA-4 from BioLegend

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FITC anti-human SSEA-4 from BioLegend

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