Fig 2: ?dT cells are found in the mouse brain after hypoxia-ischemia (HI)–induced preterm brain injury. A–D: The mRNA expression of Trg (A), Trgv4 (B), Trgv5 (C), and Trgv7 (D) in the hemisphere ipsilateral to the injury compared with the mRNA level in the normal control mice. E–Q: Presence of ?dT cells in the mouse brain after HI injury. E: ?dT-cell counts from immunohistochemical staining at 6 hours after HI. F and G: Representative immunohistochemical staining of ?dT cells at 6 hours after HI in the mouse brain in the meninges of the ventral part of the retrosplenial area (F) and in the meninges of the cortical amygdala area (G) in the ipsilateral hemisphere. H–K: ?dT cells in the contralateral (H) and ipsilateral (I) periventricular area by the anterior pretectal nucleus (APN) and lateral posterior nucleus (LPN) of the thalamus and in the contralateral (J) and ipsilateral (K) meninges by the cortical amygdala area (COA). L–Q: In eGFP ?dT-cell reporter mice, eGFP+ ?dT-cells are shown in the meninges by the cortical amygdala area in the ipsilateral hemisphere at 6 hours after HI. L: Enhanced green fluorescent protein–positive (eGFP+) ?dT cells. N: Nuclear staining with DAPI. P: Overlay of eGFP+ ?dT cells and DAPI. M, O, and Q: Higher-magnification images of L, N, and P, respectively. Data are expressed as means ± SEM of the ratio of the target gene to the reference gene Rna18s1 (A–D) and means ± SEM ?dT-cell counts (E). n = 6 to 8 (A–D, HI mice); n = 5 to 6 (A–D, controls); n = 8 (E, HI mice). *P < 0.05, **P < 0.01, and ***P < 0.001 versus control mice (n = 8); †P < 0.05 between the contralateral and the ipsilateral hemispheres. Scale bars = 10 µm (F and G); 50 µm (H–L, N, and P); 20 µm (M, O, and Q).