Fig 2: Binding analysis of MMINA with COX2, STAT3, TNF-α, NF-κB and p65protein. In the 3D confirmation, protein is shown in snake ribbon while the MMINA is shown in grey ball and stick model. In 2D representation blue ball and stick model represent the MMINA, residues involved in hydrophobic interactions are shown in pink disc while the residues involved in covalent and coordinate covalent interactions are shown in green discs. orange lines indicating pi-interactions in p65.

Fig 3: Western blot analysis of STAT3, COX2, NFĸB p65, IL1 and TNF-α protein expression in (a) Kidney, (b) Liver, (c) Brain and (d) Heart tissue lysates from different treatment groups. Treatments are indicated above the blots. Full sized blots are included in the Supplementary file S3. All gels and blots were run under the same experimental conditions as described in “Methods”.

Fig 4: Quantification of mRNA expression of inflammatory mediators (COX-2, IL1 and iNOS) in various organs. *, **, ***, **** indicated p < 0.05, p < 0.01, p < 0.001 and p < 0.0001 versus saline group respectively, and ++++P < 0.0001 versus CDDP (12 mg/kg group). Statistics was carried out by one-way ANOVA followed by the Tukey- post-test. Each value indicates the mean ± SEM (n = 7).

Fig 5: Modeling of three-dimensional structures for rat proteins. (A) 3D image of COX2. (B) Ramachandran plot of COX2. (C) 3D image of GPx. (D) Ramachandran plot of GPx. (E) 3D image of IL-1. (F) Ramachandran plot of IL-1. (G) 3D image of SOD. (H) Ramachandran plot of SOD. (I) 3D image of STAT3. (J) Ramachandran plot of STAT3. (K) 3D image of NF-κB. (L) Ramachandran plot of NF-κB. (M) 3D image of P65. (N) Ramachandran plot of P65. (O) 3D image of TNF-α. (P) Ramachandran plot of TNF-α.