Fig 1: HBV-STAT3-SALL4 axis inhibits miR-200c transcription. a The mRNA and protein levels of SALL1, SALL2 and SALL4 of HBV+HLCZ01 cells were determined by q-RT-PCR (upper) and western Blot (below). b miR-200c expression was determined in HBV+HLCZ01 cells transfected with si-SALL1, si-SALL2, and si-SALL4 (left), miR-200c expression was determined in HBV+HepaRG cells transfected with si-SALL4 (right). c PD-L1 expression was assayed by FACS in HBV+HLCZ01 cells transfected with si-SALL4. d miR-200c expression was determined in HBV+HLCZ01 cells transfected with SALL4 over-expressing vector. e PD-L1 expression was assayed in SALL4 over-expressed HBV+HLCZ01 cells. f The mRNA level of SALL4 in HBV+HepaRG cells was determined by q-RT-PCR. g PD-L1 expression was assayed by FACS in HBV+HepaRG cells transfected with si-SALL4. h PD-L1 expression was assayed by FACS in HBV+HepaRG cells transfected with SALL4 over-expressing vector. i The activity of the miR-200c promoter reporter was assayed. j ChIP with SALL4 antibody showed binding of SALL4 to the miR-200c promoter in the HBV-infected H LCZ01 cells. Data are representative of three independent experiments. Significant differences calculated using unpaired two-tailed Student’s t-tests. *P < 0.05, **P < 0.01