LAMP-1/CD107a Antibody (5H6) NBP2-25154 from Novus Biologicals, a Bio-Techne Brand

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Nature communications
May 22 2018

Fig 1: D2.0 R cells activate autophagy upon entering a dormant state. Representative images are shown for a Immunofluorescent staining of lysosomal (LAMP1, red) and autophagic (LC3, green) markers of D2.0 R cells in BME (upper panels) and BME + COL1 (lower panels) with b Quantification of the percentage of cells exhibiting LC3 positive puncta and LAMP1 staining out of the total number of cells analysed (mean ± s.e.m, n = 30–42 cells. Comparisons are relative to Day 1 by Kruskal–Wallis, Dunn’s post test. *P = 0.05; ****P = 0.0001) and the average number of LC3 puncta per cell (Comparisons by Kruskal–Wallis, Dunn’s post test. **P = 0.01 and ****P = 0.0001). c D2.0 R cells transfected with the mCherry-GFP-LC3 reporter show activation and completion of the autophagic cycle (red fluorescence only) when plated in BME matrices for 8 days. d The graphs represent the percentage of cells with double-positive puncta (mCherry+ and GFP+) (autophagosomes) out of the total number of cells analysed (left graph) (mean ± s.e.m, n = 35–39 cells). Comparisons by Kruskal–Wallis, Dunn’s post test. ****P = 0.0001) and the rate of conversion from cells exhibiting yellow puncta to red-only puncta expressed as fold change (right graph). Scale bars are 50 and 10 µm for a and 20 and 10 µm for b
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Metabolism open
December 01 2021

Fig 2: Evaluation of autophagic activity following SREBP1c silencing and OA administrationCells were transfected with siRNA specific for SREBP1c and then treated with OA:BSA complex (0.5 mM) for 24 h to divide into following groups; i) control, ii) OA, iii) SREBP1c siRNA, iv) SREBP1c siRNA + OA. Representative BODIPY/LAMP1 co-stained confocal microscopic images from each group. Cells were fixed, and then stained for BODIPY (green), LAMP1 (red) and nuclei by DAPI (blue). The graph shows quantification of the numbers of lipid droplet co-localized with LAMP1 per cell as explained in Methods section (A). Protein expressions of each group were measured by western blotting following the normalization to ß-actin against Beclin 1 (B) and LC3 antibodies (C). Scale bar = 10 µmData are expressed as mean ± S.D.**p < 0.01, and *p < 0.05 vs. control,#p < 0.05 vs. OA, (n = 3). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)