Fig 1: Autoantigens were translocated into apoptotic bodies (ABs). (a) ABs isolated from 0.8 mM H2O2 for 24 h treated cells were harvested and resuspended in lysis buffer. In parallel, lysates of apoptotic cells were analyzed. Then, Western blot analyses were performed as described in Materials and Methods. The detected vitiligo antigen proteins were indicated in the figure. (b) TRRP-1 and Lamin A/C (Asp230) were detected the colocalization with ABs through the immuno-electron microscope. (c) The distribution of subcellular localization of AB antigens before blebs was identified by Western blot. H1, H2A, La/SSB, HSP 70, and HSP90 were detected in ABs. (d, e) TRRP-1 and Lamin A/C (Asp230) were evaluated in lysates of ABs at 0, 0.2, 0.4, 0.8, and 1.0 mM of H2O2 treatment of PIG3V cell. Then, lysates of ABs were prepared after the indicated incubation periods, and antigens were detected by Western blot analysis. The upper Western blot shows the corresponding internal reference protein ß-actin.