Fig 1: Validation of sT protein interaction with chromatin binding and gene-specific transcription factor proteins identified by Bio-ID.(A) Confirmation of LTSD-dependent direct sTWT interaction with Cux1, c-Jun, CBP, and BRD9 by immunoprecipitation (IP). N-terminally SF-tagged (NSF) NSF-sTWT and NSF-sTLTSD cell lysates were immunoprecipitated with FLAG Ab. 2.5% of IP lysate was used as input. SF-sT was pulled down to confirm equal precipitation of sTWT and sTLTSD. PP2Ac, which interacts with both sTWT and sTLTSD, was used as a positive control. (B) MCV?sT transfected 293 TRE NSF-sTWT and NSF-sTLTSD cells were treated with Dox at day 1 p.t. and harvested day 4 p.t. for immunofluorescence. Cells were co-stained with antibodies against FLAG sT (red, Alexa 488) and either Cux1, c-Jun, or CBP (green, Alexa 568), and images were captured by confocal microscopy. Magnification 100x. Arrowheads designate sT-positive mitotic cells. White bars on DAPI images indicate 10 µm.