Fig 2: Suchilactone regulated the proliferation and apoptosis of AML cells by inhibiting the activation of SHP2. SHI-1 cells were seeded in 6-well plates for 12 h and then treated with different concentrations of suchilactone or 0.1% DMSO for 24 h. (A and B) Western blot was used to detect the activation of SHP2, ERK pathway, and apoptosis-related proteins. (C and D) Real-time quantitative PCR was used to detect the mRNA expression level of apoptosis-related genes. The experiment was repeated three times. Data are shown as the means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.00001 vs. the DMSO control.

Fig 3: Verification of interaction between suchilactone and SHP2. The 3D structure of SHP2 was downloaded from PDB (PDB ID: 5EHR), and the structures of suchilactone were downloaded from PubChem. The docking process was conducted in Autodock4 with a coarse docking using a simulated annealing algorithm and a subsequent refinement using a genetic algorithm, which were set to run 100 and 50 times, respectively. (A) Structural diagram of interaction between suchilactone and SHP2. (B) Binding site of suchilactone on SHP2. The SHI-1 cells were seeded in 10 cm plates for 12 h and then treated with suchilactone or 0.1% DMSO for another 4 h. (C) CETSA was used to verify the interaction between suchilactone and SHP2. (D) The relative ratio of SHP2 was quantified between the two groups, the experiment was repeated three times.