Fig 2: Circ-CTNNB1 promotes aerobic glycolysis in OS. (A, B) Seahorse tracing curves (A) and ECAR and OCR (B) of 143B cells stably transfected with sh-Scb and sh-circ-CTNNB1#1, #2 or treated with glucose (10 mM), oligomycin (2 µM), or 2-deoxyglucose (2-DG, 50 mM) at indicated points. (C–E) Glucose uptake (C), lactate production (D), and ATP levels (E) in 143B and MG-63 cells stably transfected with sh-Scb and sh-circCTNNB1#1, #2. (F) Venn diagram overlapping RBM15 CLIP-seq with glycolytic genes revealing five target genes involved in the aerobic glycolysis of the circ-CTNNB1/RBM15 axis. (G–I) Transcript and protein expression levels of ALDOA, ENO1, GPI, HK2, and PGK1 in 143B cells with overexpression or knockdown of RBM15 and circ-CTNNB1 measured by qRT-PCR (G, H) and western blot (I) assay. (Data were mean ± SEM of three experiments. Fisher's exact test for over-lapping analysis in F, Student's t-test and ANOVA analysed the difference in A–E, G, H. *p < 0.05 vs. sh-Scb, mock or circ-Mock)