Fig 1: Effect of titanium-enriched medium on TETs. Firstly, the gene activities were evaluated by qPCR, as follows: TET1 (a), TET2 (b), and TET3 (c), where GADPH gene was used to normalize the data as a housekeeping gene. Thereafter, the protein profile was evaluated by Western blot by resolving 75 µg of sample per lane and later incubating it with specific antibodies. Representative immunoblots of TET1 (d) and TET2 (e) are presented. Densitometric analysis of immunoblots was normalized to the protein ratio of controls and GAPDH, here used as loading control (housekeeping control). Differences were considered statistically significant when * p < 0.04, ** p < 0.001, **** p < 0.0001 when compared with the control (Ctrl) group; and * p < 0.04 when comparing wo_Ti and w_Ti. The graphs show mean ± standard deviation of three independent experiments. Note: TET: ten-eleven translocation methylcytosine dioxygenase; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; Ctrl: control group; wo_Ti: medium enriched with titanium without surface modification; w_Ti: medium enriched with titanium with surface modification. The experiment was performed in triplicate (n = 3).