Fig 1: Colocalization of various Pericentrin interaction partners at the basal body complex of mouse photoreceptors.(A) Scheme of a vertebrate rod photoreceptor. (B–F) Micrographs of a vertical cryostat section through an adult mouse retina double labeled for CG-NAP (B, green) and Cen3 (C, red) as a marker for the ciliary apparatus (the connecting cilium (CC) and the basal body complex (BBC)) and the centrosomes. (B) CG-NAP and (C) Cen3 are localized in the ciliary region of the photoreceptors and at the centrosomes of the other retinal cells. (D) The merge of the stainings with the additional DAPI nuclear staining (blue) shows the partial colocalization of CG-NAP and Cen3 in the ciliary region of the photoreceptors and the centrosomes of the other retinal cells. (E–F) High power views showing the localization of CG-NAP at the BBC of the connecting cilium (E) and at the centrosomes of the other retinal cells (F). (G) Scheme of the ciliary apparatus. (H–K) High power views showing the localization of the Pcnt interaction partners PCM1 (H, green), DISC1 (I, green), IFT20 (J, green), and PC2 (K, green) at the BBC of the connecting cilium of the photoreceptor. Cen3 (red) marks the whole cilium with the ciliary apparatus. RPE: retinal pigment epithel; OS: outer segment; IS: inner segment; ONL: outer nuclear layer; OPL: outer plexiform layer; BB: basal body; CC: connecting cilium; C: centriole; CP: calycal process; MTs: microtubules; N: nucleus; S: synapse. Scale bars: 10 µm (B), 1 µm (E, F, J, K, H, I).