Fig 2: Downregulation of RSRC1 is associated with a poor prognosis of patients with GC. (A) Pie chart showing that RSRC1 expression is downregulated in the majority of the tumor tissues compared to the paired adjacent normal tissues. According to the fold change (T/N) of RSRC1 mRNA expression in each pair of samples, the results of RT-qPCR were classified as upregulated (>2.0), stable (0.5–2.0) and downregulated (<0.5). (B) Representative images of immunohistochemistry showing the protein expression of RSRC1 in normal tissues compared to tumor tissues. Magnification, ×40 (insert) and ×200. (C) Kaplan Meier curves comparing the overall survival (OS) of patients with tumors with a low expression of RSRC1 (black line; n=370) and a high RSRC1 expression (red line; n=261), P<0.01, log-rank test. T, tumor tissue; N, adjacent normal tissue; RSRC1, arginine/serine-rich coiled coil 1; GC, gastric cancer.

Fig 3: RSRC1 regulates PTEN expression in GC cells. (A) Western blot analysis was performed to detect the expression levels of specific proteins involved in signaling pathways related to tumor development after the silencing of RSRC1 expression in AGS cells. (B) RT-qPCR was performed following RSRC1 knockdown in SGC7901 and AGS cell lines to observe the alterations in PTEN mRNA expression. RSRC1, arginine/serine-rich coiled coil 1; GC, gastric cancer.

Fig 4: Knockdown of RSRC1 promotes GC cell migration. (A) Cell migratory ability was measured by Transwell migration assays following transfection. Representative images of migrated cells are shown, and all the experiments were repeated at least 3 times independently. Magnification, ×100. (B) The number of migrated cells was counted in 5 randomly selected microscopic fields and the data are presented as the means ± SD. *P<0.05 and **P<0.01 vs. NC group. RSRC1, arginine/serine-rich coiled coil 1; GC, gastric cancer.