Fig 2: Redox states of the ?-subunit of ATP synthase (CF1?), phosphoribulokinase (PRK) and 2-Cys peroxiredoxins (2-Cys PRX) in dark-adapted plants and plants illuminated under various irradiances.Leaves from 35-day-old Col-0, ntrc and transgenic lines overexpressing (OE) modified TRX systems (OE-NTRC, OE-NTR-TRXf, and OE-SGPS) were collected at the end of the night (D), or from plants illuminated for 2 h at 50 (low light, LL), 200 (growth light, GL) and 800 (high light, HL) µmol photons m-2 sec-1 and frozen in liquid nitrogen. Total leaf proteins were extracted and separated in sodium dodecyl sulphate–polyacrylamide gel electrophoresis as described in Figure 1 before Western blotting with anti-2-Cys Prx antibody, anti-Cf1? antibody and anti-PRK antibody. Bands corresponding to the oxidized (Ox) and reduced (Red) form of the proteins are marked in the figure. 2-Cys PRX exist as three redox forms, fully oxidized (Ox), partially reduced (1SH) and fully reduced (2SH) (Nikkanen et al., 2016). Ponceau staining was used as the loading control.