Fig 1: Ser177 phosphorylation of Pdlim5 disrupts its association with Arhgef6 at the cell periphery.(a) Immunoblotting analysis of GST-Pdlim5 pull-down assay. Eluates were subjected to immunoblotting with anti-Arhgef6 antibody. Coomassie staining demonstrates equal loading of GST-Pdlim5 proteins. (b) Immunostaining images of Arhgef6 and Pdlim5 from KDR/EGFP-Pdlim5 cells. Boxed area in KDR/EGFP-WT-Pdlim5 cell highlights representative co-localization of Arhgef6 with Pdlim5 at the cell periphery. Scale bars, 10 µm. (c) Immunostained images of Arhgef6 knockdown vSMCs stained with anti-Pdlim5 antibody, anti-Arhgef6 antibody and phalloidin. Scale bars, 10 µm.