Fig 1: Distribution of STIM1 and ORAI1 and their complexes in WT and CPVT myocytes. (A) Representative images of WT (top) and CPVT (bottom) myocytes immunostained for STIM1. Scale bars are 10 µm. (B) Summary graph of ratios of myocyte end to - center STIM1 immunofluorescence for WT and CPVT myocytes (as indicated). Region-specific STIM1 immunofluorescence intensities were measured in 4–5 areas at either myocyte ends or interior. (C) Representative images of WT (top) and CPVT (bottom) myocytes immunostained for ORAI1. Scale bars are 10 µm. (D) Summary graph of ratios of myocyte end to - center ORAI1 immunofluorescence for WT and CPVT myocytes (as indicated). (E) Representative images of WT (top) and CPVT (bottom) myocytes subjected to proximity ligation assay (PLA) for STIM1 and ORAI1 co- localization. Scale bars are 10 µm. (F) Summary graph of ratios of myocyte end to - center STIM1-ORAI1 PLA signal densities for WT and CPVT myocytes (as indicated). Region-specific immunofluorescence intensities (PLA signal densities) were measured as number of signals per cell. Data presented as mean ± SE from 15 myocytes from 4 animals.