Cytochrome c (6H2.B4) Mouse mAb 12963S from Cell Signaling Technology

Product Specs
ItemCytochrome c (6H2.B4) Mouse mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number12963S
Quantity100 µl
ApplicationsIP, IF-IC
ReactivityH, M, R
Targetcytochrome C
HostMouse
NCBI Full Gene Namecytochrome c, somatic
NCBI Gene AliasesCYC, HCS, THC4
ClonalityMonoclonal
Research AreaApoptosis
Clone6H2.B4
Molecular Weight14

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Silver nitroprusside as an efficient chemodynamic therapeutic agent and a peroxynitrite nanogenerator for targeted cancer therapiesJournal of advanced researchFebruary 1, 2024Asif K, Adeel M, Rahman MM, Sfriso AA, Bartoletti M, Canzonieri V, Rizzolio F, Caligiuri I.1% BSA/PBS, at 4 °C, overnight) purchased from Cell Signaling Technology, Danvers, MA, USA (Cat. # 12963), followed by a secondary antibody staining (1: 1000 dilution, Alexa Fluor® 488 dye) for 2 h at room

(2) Endoplasmic Reticulum Stress Disrupts Mitochondrial Bioenergetics, Dynamics and Causes Corneal Endothelial Cell ApoptosisInvestigative Opthalmology & Visual ScienceNovember 14, 2023Saba Qureshi, Stephanie Lee, William Steidl, Lukas Ritzer, Michael Parise, Ananya Chaubal, Varun KumarGermany). A similar immunocytochemistry method was used for staining mitochondria by cytochrome C (cat no. 12963, 1:1000; Cell Signaling) except for treatment (0.2%, 0.4% DMSO; 10, 20 µg/mL tunicamycin for six hours),

(3) Enhanced Efficiency of the Basal and Induced Apoptosis Process in Mucopolysaccharidosis IVA and IVB Human FibroblastsInternational Journal of Molecular SciencesSeptember 14, 2023Joanna Brokowska, Lidia Gaffke, Karolina Pierzynowska, Grzegorz Węgrzyntemperature, and subsequently incubated overnight with anti-cytochrome c antibody, diluted 1:600 (#12963; Cell Signaling Technology Inc., Danvers, MA, USA). Afterward, secondary antibody, conjugated with Alexa

(4) A druggable copper-signalling pathway that drives inflammationNatureMay 1, 2023Solier S, Müller S, Cañeque T, Versini A, Mansart A, Sindikubwabo F, Baron L, Emam L, Gestraud P, Pantoș GD, et al.GR320655-1, FM 1:400, Hu), CREM (Proteintech, 12131-1-AP, WB 1:1,000, Hu), cytochrome c (Cell Signaling, 12963S, clone 6H2.B4, lot 1 and 2, FM 1:400, NS 1:400, Hu), DMT1 (Abcam, ab55735, clone 4C6, lot GR3243346-1,

(5) Heightened apoptotic priming of vascular cells across tissues and life span predisposes them to cancer therapy-induced toxicitiesScience advancesNovember 11, 2022Spetz JKE, Florido MHC, Fraser CS, Qin X, Choiniere J, Yu SJ, Singh R, Friesen M, Rubin LL, Salem JE, et al.SDS–polyacrylamide gel electrophoresis and Western blot for anti–cytochrome c (Cell Signaling Technology, #12963). Western blotting Western blotting was performed as previously described (13). Briefly, the polyvinylidene

(6) Vitamin C inhibits apoptosis in THPâ€‘1 cells in response to incubation with Mycobacterium tuberculosisExperimental and Therapeutic MedicineOctober 11, 2022Fuyang Song, Yiming Wu, Xue Lin, Di Xue, Yujiong Wangno. 20750), Cleaved-caspase-3 (cat. no. 9661), Bcl-2 (cat. no. 15071), cytochrome c (Cyt-c; cat. no. 12963), Bax (cat. no. 5023), IL-1β (cat. no. 12703), IL-6 (cat. no. 12912) or NLR family pyrin domain-containing

(7) Arabic Gum Could Alleviate the Aflatoxin B1-provoked Hepatic Injury in Rat: The Involvement of Oxidative Stress, Inflammatory, and Apoptotic PathwaysToxins (Basel)September 1, 2022Noha Ahmed et al.Factor Nrf2,SANTA CRUZ,Sc-518036,H-10,1/1000 SOD1,SANTA CRUZ,Sc-101523,24,1/1000 Cytochrome c,Cell signaling,12963S,6H2.B4,1/1000 IL-6,Cell signaling,12912,D5W4V,1/1000 Caspase3,Cell signaling,#9662,,1/1000 Cleaved-Caspase3

(8) Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signalingImmunityAugust 9, 2022Xian H, Watari K, Sanchez-Lopez E, Offenberger J, Onyuru J, Sampath H, Ying W, Hoffman HM, Shadel GS, Karin M.Caspase-3 (Asp175),Cell Signaling,Cat#9661; RRID: AB_2341188 Cytochrome c (6H2.B4),Cell Signaling,Cat#12963; RRID: AB_2637072 Brilliant violet 510™ anti-mouse CD45,BioLegend,Cat#103137; RRID:AB_2561392 "Anti-rabbit

(9) Confined migration promotes cancer metastasis through resistance to anoikis and increased invasivenesseLifeMarch 8, 2022Fanfone D, Wu Z, Mammi J, Berthenet K, Neves D, Weber K, Halaburkova A, Virard F, Bunel F, Jamard C, et al.RRID:AB_2085424",IF (1/200)WB (1/1000) Antibody,Cytochrome c (mouse monoclonal),Cell Signaling,"Cat# 12963, RRID:AB_2637072",IF (1/200) Antibody,Vinculin(mouse monoclonal),Sigma-Aldrich,Cat#V9131; RRID: AB_477629,IF

(10) Genome‐wide analysis of DNA methylation identifies the apoptosis‐related gene UQCRH as a tumor suppressor in renal cancerMolecular OncologyFebruary 1, 2022Kosuke Miyakuni, Jun Nishida, Daizo Koinuma, Genta Nagae, Hiroyuki Aburatani, Kohei Miyazono, Shogo Ehatawith 0.1% Triton X‐100 containing Tween20. Then, they were stained with anticytochrome c antibody (12963; 1 : 300, Cell Signaling Technology, Danvers, MA, USA) and visualized using anti‐mouse IgG H&L secondary

(11) N6-methyladenosine demethylase FTO promotes growth and metastasis of gastric cancer via m6A modification of caveolin-1 and metabolic regulation of mitochondrial dynamicsCell Death & DiseaseJanuary 21, 2022You Zhou, Qi Wang, Haifeng Deng, Bin Xu, Yi Zhou, Jian Liu, Yingting Liu, Yufang Shi, Xiao Zheng, Jingting Jiang(1:1000, Cat#11925), p-Parkin1 (1:1500, Cat#36728), Parkin1 (1:1000, Cat#2131), Cytochrome c (1:2000, Cat#12963) and β-tubulin (1:1500, Cat#2146) were obtained from Cell Signaling Technology. Antibody against p-MFN2

(12) Engineered virus-like particles for efficient in vivo delivery of therapeutic proteinsCellJanuary 20, 2022Banskota S, Raguram A, Suh S, Du SW, Davis JR, Choi EH, Wang X, Nielsen SC, Newby GA, Randolph PB, et al.Kit,QIAGEN,Cat#28704 QIAGEN Plasmid Plus Midi Kit,QIAGEN,Cat#12943 QIAGEN Plasmid Plus Maxi Kit,QIAGEN,Cat#12963 FastScan Cas9 (S. pyogenes) ELISA Kit,Cell Signaling Technology,Cat#29666C MuLV Core Antigen ELISA

(13) Disrupted mitochondrial homeostasis coupled with mitotic arrest generates antineoplastic oxidative stressOncogeneJanuary 1, 2022Hao X, Bu W, Lv G, Xu L, Hou D, Wang J, Liu X, Yang T, Zhang X, Liu Q, et al.anti-SUMO1 (ab32058, abcam, 1:1000), anti-Cytochrome C (ab13575, abcam, 1:1000), anti-Cytochrome C (#12963, CST, 1:1000), anti-PGC-1α (66369-1-Ig, proteintech, 1:1000), anti-p-AKT-Ser473 (#4060, CST, 1:1000),

(14) Aloe-Emodin Induces Mitochondrial Dysfunction and Pyroptosis by Activation of the Caspase-9/3/Gasdermin E Axis in HeLa CellsFrontiers in pharmacologyJanuary 1, 2022Li T, Shi L, Liu W, Hu X, Hui Y, Di M, Xue S, Zheng Y, Yao M, Li C, et al.Antibodies for caspase-1 (98033), caspase-3 (9662S), caspase-8 (4790S), caspase-9 (9502S), cytochrome c (12963S), Bid (2002S) and Tom20 (42406S) were from Cell Signaling Technology. Reagents were purchased as follows:

(15) Direct Cardiac Actions of Sodium-Glucose Cotransporter 2 Inhibition Improve Mitochondrial Function and Attenuate Oxidative Stress in Pressure Overload-Induced Heart FailureFrontiers in cardiovascular medicineJanuary 1, 2022Li X, Flynn ER, do Carmo JM, Wang Z, da Silva AA, Mouton AJ, Omoto ACM, Hall ME, Hall JE.Then, the sections were incubated at 4°C overnight following addition of Cyt C (1:500, Cell Signaling, 12963) and IraZolve-Mito (1:1000, Cayman, 25910). Subsequently, samples were washed three times and incubated

(16) Antioxidant and Cytoprotective Properties of Polyphenol-Rich Extracts from Antirhea borbonica and Doratoxylon apetalum against Atherogenic Lipids in Human Endothelial CellsAntioxidants (Basel)December 24, 2021Jonathan Bonneville, Philippe Rondeau, Bryan Veeren, Julien Faccini, Marie-Paule Gonthier, Olivier Meilhac, Cécile Vindisalbumin (BSA), cells were incubated with an anti-cytochrome C primary antibody (1:100 dilution, mouse mAb,12963, Cell Signaling Technology, Saint Quentin Yvelines, France) for 1 h at RT and revealed with Alexa Fluor-conjugated

(17) TRAIL sensitivity of nasopharyngeal cancer cells involves redox dependent upregulation of TMTC2 and its interaction with membrane caspase-3Redox biologyNovember 20, 2021Raman D, Tay P, Hirpara JL, Liu D, Pervaiz S.Antibody,Catalog #,Company,Application Cytochrome-C,12963,Cell Signaling Technologies,Western Blotting Bid,8762,Cell Signaling Technologies,Western Blotting Cu/Zn SOD

(18) MACF1 controls skeletal muscle function through the microtubule-dependent localization of extra-synaptic myonuclei and mitochondria biogenesiseLifeAugust 27, 2021Alireza Ghasemizadeh, Emilie Christin, Alexandre Guiraud, Nathalie Couturier, Marie Abitbol, Valerie Risson, Emmanuelle Girard, Christophe Jagla, Cedric Soler, Lilia Laddada, et al.factor,Manufacturer and reference Anti-Cytochrome C,Mouse - primary,1:300,Cell Signaling Technologies 12963S (RRID:AB_2637072) Anti-MACF1,Rabbit - primary,1:200,Novus Biologicals NBP2-36528 (RRID:AB_2893083) Anti-alpha

(19) Targeting the IDO-BCL2A1-Cytochrome c Pathway Promotes Apoptosis in Oral Squamous Cell CarcinomaOncoTargets and TherapyMarch 4, 2021Qiaoping Zheng, Guifang Gan, Xianfu Gao, Qingqiong Luo, Fuxiang Chento reduce nonspecific labeling. The fixed slides were stained with a primary anti-cytochrome c antibody (12963S; Cell Signaling Technology, Danvers, MA, USA) at 4°C for 12–16 hours, then with an AF-561-labeled secondary

(20) Astragaloside IV inhibits palmitic acid-induced apoptosis through regulation of calcium homeostasis in mice podocytesMolecular Biology ReportsFebruary 19, 2021Yingjun Zang, Shuang Liu, Aili Cao, Xiangyu Shan, Wenjuan Deng, Zhijun Li, Hao Wang, Yunman Wang, Li Wang, Wen Pengovernight at 4 °C with primary antibody nephrin (ab216341, Abcam, Cambridge, MA, USA), cytochrome c (12963S, Cell Signaling, Beverly, MA) or TRPC6 (ab62461, Abcam, Cambridge, MA, USA) diluted in PBST and then

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Cytochrome c (6H2.B4) Mouse mAb from Cell Signaling Technology

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Cell Signaling Technology

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Cytochrome c (6H2.B4) Mouse mAb from Cell Signaling Technology

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