Fig 1: LEFTY1 and OLFM4 are mainly expressed in Barrett’s oesophagus cells that do not express differentiated secretory cell markers. a Upper panel, cluster consensus matrix of BO cells from 4 BO patients (n = 371 cells). Blue-to-red colours denote the frequency with which cells are grouped together in 250 repeat clusterings of simulated technical replicates (see Methods). Clusters (B1-B4) are indicated by the coloured bars below. Lower panel, heatmaps showing expression of selected functionally relevant genes that are differentially expressed between cell clusters (>4 fold change, FDR <1e-5). b Immunohistochemical staining of MUC2, LEFTY1 and CHGA in sections derived from the same BO resection specimen. Black arrows indicate goblet cells on all sections (positively stained for MUC2; negative for LEFTY1 and CHGA). Scale bars are 50 µm. c Immunohistochemical staining of LEFTY1 in an OSG from a normal squamous endoscopic biopsy obtained from a patient with BO. Scale bars are 300 µm and 50 µm in enlarged image