Fig 1: Gating strategy for analysis of extracellular vesicles (EVs). Samples were marked with tag-it-violet (TIV) to identify EVs. Anti-MCP1, p16, uromodulin, podocalyxin, prominin-2, and URAT-1 antibodies were added together. Gates were selected starting from the TIV + population, after running a sample of unlabeled vesicles and compared with a single-stained sample for each antibody. Imaging flow cytometry corroborated the gateways made by observing the running events. BF indicates bright field; MCP1, monocyte chemoattractant protein-1; SSC, side scatter; URAT-1, urate transporter 1.