Fig 1: Disruption of the OLM and to a lesser extent the ILM in Pten cKO retinas. (A-L') Immunolabelling of P21 wild-type and Pten cKO retinas for ZO-1 (A-B'), N-cadherin (C-D'), Otx2 (E-F'), RPE65 (G-H'), laminin (I-J') and fibronectin (K-L'). Blue is DAPI counterstain. Insets in A-L' are 2.5-fold magnifications of the boxed areas in the larger images. Loss of integrity of the OLM in the Pten cKO peripheral retina and hamartoma is indicated with asterisks in B',D'. Scale bars: 200 µm. (M-O') Electron microscopy of P21 wild-type (M,M') and Pten cKO retinas in the periphery (N,N') and hamartoma region (O,O'), focusing on the ILM. M'-O' are 2.5-fold magnifications of the boxed areas in M-O, respectively. Insets in M'-O' are 2-fold magnifications of boxed areas in M'-O'. gcl, ganglion cell layer; ham, hamartoma; ilm, inner limiting membrane; inl, inner nuclear layer; ld, lamina densa; lre, lamina rara externa; lri, lamina rara interna; NR, neural retina; onl, outer nuclear layer; rpe, retinal pigment epithelium.