Fig 1: Endothelial differentiation of cancer stem cells of human colorectal carcinomas (CoCSC) in vitro. (a) Representative immunofluorescence micrographs of CoCSC spheres sections labeled with CD31 and NuMA (i–iii), CD144 and CDX2 (iv–vi) or VEGFR2 and NuMA (vii–ix) showed that CD31 and VEGFR2 were negative but CD144, NuMA and CDX2 were positive in CoCSC sphere. (b) Representative immunofluorescence micrographs of CoCSC cultured in DFS showed that the CD31 (i) and VEGFR2 (vii) were negative but the CD144 (iv), CDX2 (viii), CK20 (ii) and NuMA (v) were positive. (c) Representative immunofluorescence micrographs of CoCSC cultured in EGM2 showed that the CD31 (i), CD144 (ii) and VEGFR2 (iv) were positive. Scale bars: 50 µm.