Fig 1: Calcitriol administration modulated the antigen-presenting function and Th2 or Th17 promoter expression in DCs. A, Representative flow cytometry plots of CD86+/MHC?+ DCs following incubations in various conditions (control group, LPS group and LPS + Cal group). B, Quantification of the proportion of CD86+/MHC?+ DCs (assessed by flow cytometry). C, mRNA levels of Th2 promoters (such as OX-40L and CCL17) in DCs following incubation in various conditions (control group, LPS group and LPS + Cal group). D, Protein levels of Th2 promoters (such as OX-40L and CCL17) in DCs (determined by Western blotting) following incubations in various conditions (control group, LPS group and LPS + Cal group; left panel) and semi-quantitative analysis of the protein expression levels (normalized to the level of actin) in terms of the relative grey density (right panel). E, mRNA levels of Th17 promoters (such as IL-23 and IL-6) in DCs following incubations in various conditions (control group, LPS group and LPS + Cal group). F, Protein levels of Th17 promoters (such as IL-23 and IL-6) in DCs (determined by Western blotting) following incubations in various conditions (control group, LPS group and LPS + Cal group; left panel) and semi-quantitative analysis of the protein expression levels (normalized to the level of actin) in terms of the relative grey density (right panel). The data are shown as the mean ± SD; *P < .05 and **P < .01 represent significant differences between the indicated columns