Fig 1: Dexamethasone upregulates the expression of the CD163 scavenger receptor. (A) a–human CD163 mAb-APC (red) and Höchst (blue) were applied for visualization purposes. HCS was performed and 3D image analyses done on GM- and Dex-MDMs. (B) Graph depicting upregulated expression of CD163 following 7-day treatment of GM-MDMs. Bars represent mean fluorescence intensity ± SD of n = 4 donors. The x-axis represents treatment conditions. GM-MDMs were treated with Dex for 24h post-differentiation period (7 days) and were repolarized into anti-inflammatory phenotypes depicted by upregulated expression of CD163 (C) and decreased expression of CD86 (D). Graphs demonstrate repolarization in three independent donors. Values are expressed as MFI ± SD, p-values indicated were calculated by one-way ANOVA.