Fig 1: Metabolic inhibitor treatments impact Th1/Th2 polarization. CD4 T cells from healthy donors were treated with various inhibitors overnight on day 11 or 12 post-activation. Cells were restimulated with PMA/ionomycin for 4 h and subjected to intracellular cytokine staining for (A) IL-2, ***p < 0.0001 (B) IFN-?, *p = 0.0415 (C) IL-4, **p = 0.0089. (D) IL-21, **p = 0.0034 (E) IL-9, and (F) IL-17. The percentage of positive cells for each cytokine was quantified by flow cytometry and plotted for multiple donors. Statistical significance was determined by RM one-way ANOVA with Dunnett's multiple comparisons test. (G) CD4 T cells from healthy donors as in (A–F) were restimulated with PMA/ionomycin for 4 h and subjected to qRT-PCR. Expression data of each transcription factor was normalized to expression of the 18s rRNA housekeeping gene. All groups were then compared to DMSO-treated T cells that had not been restimulated to calculate fold changes. Statistical significance was determined by two-way ANOVA with Dunnett's multiple comparisons test. **p = 0.0066.