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Anti-TLR3 antibody
Anti-TLR3 antibody from Abcam
Be the first to write a review!
Citations:
(41)
Supplier Page
Supplier Page from
Abcam for
Anti-TLR3 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-TLR3 antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab62566
Antibody Type
Primary
Isotype
IgG
Host
Rabbit
Clonality
Polyclonal
Quantity
100 UG
Applications
Immunocytochemistry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/Tag
Unconjugated
Concentration
1 mg/ml
Research Area
Immuno-oncology
Gene Name
TLR3
Reactivity
Human, Mouse
Target
TLR3
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (41)
(1)
Extracellular RNAs-TLR3 signaling contributes to cognitive impairment after chronic neuropathic pain in mice
Signal Transduct Target Ther
August 7, 2023
Xueying Zhang, Rui Gao, Changteng Zhang, Yi Teng, Hai Chen, Qi Li, Changliang Liu, Jiahui Wu, Liuxing Wei, Liyun Deng,
et al.
at 4 °C. The primary antibodies used were as follows: anti-TLR3 rabbit polyclonal antibody (
1:200
,
ab62566
,
Abcam
), anti-MAP2
chicken
antibody (
1:200
,
ab5392
,
Abcam
), anti-NeuN
mouse
antibody (
1:100
, 26975,
(2)
High expression of TLR3 in triple-negative breast cancer predicts better prognosis—data from the Fudan University Shanghai Cancer Center cohort and tissue microarrays
BMC Cancer
April 1, 2023
Lei Fan, Xin-Yi Sui, Xi Jin, Wen-Juan Zhang, Peng Zhou, Zhi-Ming Shao
citrate buffer pH 6.0 to unmask the epitopes. Rabbit polyclonal anti-
human
TLR3 antibody (
Abcam
, #
ab62566
), the primary antibody, was diluted
1:50
in phosphate-buffered saline (PBS). The chips were incubated
(3)
A p53-TLR3 axis ameliorates pulmonary hypertension by inducing BMPR2 via IRF3
iScience
February 17, 2023
Bhagwani AR, Ali M, Piper B, Liu M, Hudson J, Kelly N, Bogamuwa S, Yang H, Londino JD, Bednash JS,
et al
.
,
Cell Signaling technology
,
2586
; RRID:AB_2160343
TLR3
,
Cell Signaling technology
,
6961
; RRID:AB_10829166
TLR3
,
Abcam
,
ab62566
; RRID:AB_956368
p53
,
Cell Signaling technology
,
2527
; RRID:AB_10695803
p53
,
Sigma Aldrich
,
MABE283
p53
,
Proteintech
,
21891-1-AP
;
(4)
Effects of bacterial and viral pathogen-associated molecular patterns (PAMPs) on multidrug resistance (MDR) transporters in brain endothelial cells of the developing human blood–brain barrier
Fluids and Barriers of the CNS
January 31, 2023
Phetcharawan Lye, Enrrico Bloise, Stephen G. Matthews
was blocked using 2% BSA for 1 h. Slides with hfBECs were incubated with primary antibodies, TLR-3 (
ab62566
,
1:500
,
Abcam
, Toronto, ON, Canada), TLR-4 (
ab22048
, 1;200,
Abcam
), TLR-8 (
ab180610
,
1:200
,
Abcam
),
(5)
Photobiomodulation promotes spinal cord injury repair by inhibiting macrophage polarization through lncRNA TUG1-miR-1192/TLR3 axis
Cellular & molecular biology letters
January 19, 2023
Ju C, Ma Y, Zuo X, Wang X, Song Z, Zhang Z, Zhu Z, Li X, Liang Z, Ding T,
et al
.
4 °C. Primary antibodies:
iNOS
(cat. no.
13120
, Cell Signaling Technology,
1:1000
), TLR3 (cat. no.
ab62566
,
Abcam
,
1:1000
), p-NF-κB (cat. no.
3033
,
Cell Signaling Technology
,
1:1000
), and β-actin (cat. no.
66009-1-IG
,
(6)
Polyinosinic:polycytidylic acid aggravates calcipotriol-induced atopic dermatitis-like skin lesions in mice by increasing the expression of thymic stromal lymphopoietin
Annals of translational medicine
February 1, 2022
Wan H, Yang H, Wei M, Chen W.
constantly shaken with primary antibodies specific to β-actin (
HRP-60008
,
Proteintech
, IL, USA), TLR3 (
ab62566
,
Abcam
, Cambridge, MA, USA), IL-13Rα1 (
ab79277
, Abcam, Cambridge, MA, USA), or
TSLP
(
ab115700
, Abcam,
(7)
Toll-Like Receptor- and Protein Kinase R-Induced Type I Interferon Sustains Infection of Leishmania donovani in Macrophages
Frontiers in Immunology
January 28, 2022
Bruna T. Dias, Amy Goundry, Aislan C. Vivarini, Tatiana F. R. Costa, Jeremy C. Mottram, Ulisses G. Lopes, Ana Paula C. A. Lima
preparation for IFA as above. Antibody dilutions were as follows: rabbit monoclonal anti-TLR3 (
Abcam
,
ab62566
) at
1:200
, rabbit monoclonal anti-IRF7 (
Abcam
,
ab62505
) at
1:100
, and the Cy3-goat anti-rabbit IgG secondary
(8)
Study on the Mechanism of Platelet-Released Clusterins Inducing Restenosis after Carotid Endarterectomy by Activating TLR3/NF-κb p65 Signaling Pathway
Journal of Healthcare Engineering
January 10, 2022
Qingyu Meng, Xichun Li, Mingyu Zhao, Shusen Lin, Xiangwen Yu, Guanglong Dong
target protein was transferred to the PVDF
membrane
by the semiwet transfer method. TLR3 (
1 : 2000
,
ab62566
,
Abcam
), NF-kBP65 (
1 : 2000
,
ab16502
,
Abcam
), and GAPDH (
1 : 5000
,
ab8245
,
Abcam
) antibody dilutions
(9)
New insights of necroptosis and immune infiltration in sepsis-induced myocardial dysfunction from bioinformatics analysis through RNA-seq in mice
Frontiers in cellular and infection microbiology
January 1, 2022
Du Y, Zhong Y, Ding R, Wang X, Xia F, Zhang Q, Peng Q.
Proteintech,
Abcam
,
ab108333
), anti-IL-1β antibody (
1:500
, affbiotech, AF5103), and anti-TLR3 (
1:500
,
Abcam
,
ab62566
). After washing, the blots were incubated with the following HRP-conjugated secondary antibodies: Goat
(10)
Conjunctival Expression of Toll-Like Receptor 3 Plays a Pathogenic Role in the Formation of Ultraviolet Light–Induced Pterygium
Investigative Opthalmology & Visual Science
August 9, 2021
Chun-Chieh Lai, Sung-Huei Tseng, Sheng-Min Hsu, Yin-Ting Huang, Chi-Chang Shieh
at room temperature. The samples were then reacted with rabbit polyclonal antibody anti-TLR3 (
1:100
,
ab62566
;
Abcam
, Cambridge, UK), anti-NF-κB (
1:100
,
ab32536
;
Abcam
), anti-p63 (
1:100
,
ab735
;
Abcam
), and anti-Ki-67
(11)
Immunohistochemical Analysis of Toll-Like Receptors, MyD88, and TRIF in Human Papillary Thyroid Carcinoma and Anaplastic Thyroid Carcinoma
Journal of Thyroid Research
July 1, 2021
Yasuhiro Nihon-Yanagi, Megumi Wakayama, Naobumi Tochigi, Fumi Saito, Hideaki Ogata, Kazutoshi Shibuya
dehydrated with ethanol, and mounted. 2.3. Antibodies Antibodies specific for
TLR2
(
ab213676
), TLR3 (
ab62566
), and TRIF (
ab13810
) were from
Abcam
. Antibodies specific for
TLR4
(
HPA049174
), TLR5 (
HPA015573
),
TLR7
(12)
In situ self-assembly of Au-antimiR-155 nanocomplexes mediates TLR3-dependent apoptosis in hepatocellular carcinoma cells
Aging (Albany NY)
January 15, 2021
Liang Yin, Weijuan Cai, Yongqian Liang, Jie Yao, Xuemei Wang, Jie Shen
performed at room temperature for 30 min, in 3 changes of 1% BSA/0.1% Tween 20/PBS. An anti-TLR3 antibody (
ab62566
,
Abcam
; Cambridge, UK), diluted
1:200
in blocking buffer, was applied overnight at 4°C. After 3 washes
(13)
Poly(I:C) preconditioning protects the heart against myocardial ischemia/reperfusion injury through TLR3/PI3K/Akt-dependent pathway
Signal transduction and targeted therapy
November 6, 2020
Chen E, Chen C, Niu Z, Gan L, Wang Q, Li M, Cai X, Gao R, Katakam S, Chen H,
et al
.
polyvinylidene difluoride
membranes
, and incubated at 4 °C for 1 h with 2 μg antibodies to TLR3 (
Abcam
,
ab62566
) followed by the addition of 15 μl of protein G magnetic beads (
Biorad
,
161-4023
). The precipitates
(14)
5-aza-2′-Deoxycytidine Induces a RIG-I-Related Innate Immune Response by Modulating Mitochondria Stress in Neuroblastoma
Cells
August 19, 2020
Hung-Yu Lin, Jiin-Haur Chuang, Pei-Wen Wang, Tsu-Kung Lin, Min-Tsui Wu, Wen-Ming Hsu, Hui-Ching Chuang
(
5321S
,
Cell Signaling
, Danvers, MA, USA),
anti-RIG-I
(
3743S
,
Cell Signaling
, Danvers, MA, USA), anti-TLR3 (
ab62566
,
Abcam
, Cambridge, UK), anti-p-IRF3 (
ab76493
,
Abcam
, Cambridge, UK), anti-
MAVS
(
sc-166583
,
Santa Cruz Biotechnology
(15)
SCIMP is a universal Toll‐like receptor adaptor in macrophages
Journal of Leukocyte Biology
February 1, 2020
Lin Luo, James E. B. Curson, Liping Liu, Adam A. Wall, Neeraj Tuladhar, Richard M. Lucas, Matthew J. Sweet, Jennifer L. Stow
Bio-Rad Laboratories
. The anti-TLR4 antibody targeting TLR4 amino acids 100-200 (
ab22048
), TLR3 antibody (
ab62566
) and TLR2 antibody (
ab16894
), used for
immunoprecipitation
and Western blotting
, were from
Abcam
(Melbourne,
(16)
BCL9 provides multi-cellular communication properties in colorectal cancer by interacting with paraspeckle proteins
Nature communications
January 7, 2020
Jiang M, Kang Y, Sewastianik T, Wang J, Tanton H, Alder K, Dennis P, Xin Y, Wang Z, Liu R,
et al
.
dilution,
MA1-25325
, ThermoFisher),
ILF2
(
1:5000
dilution,
PA5-18718
, ThermoFisher), TLR3 (
1:500
dilution,
ab62566
,
Abcam
), β-catenin (
1:1000
dilution, 610154, BD Transduction Laboratories),
CD44
(
1:1000
dilution, #
3570
,
(17)
Divergent Roles of Kupffer Cell TLR2/3 Signaling in Alcoholic Liver Disease and the Protective Role of EGCG
Cellular and Molecular Gastroenterology and Hepatology
January 1, 2020
Pingping Luo, Fei Wang, Nai-Kei Wong, Yi Lv, Xinxin Li, Mianhuan Li, George L. Tipoe, Kwok-Fai So, Aimin Xu, Shuaiyin Chen,
et al.
ab133504
), Fas-associated death domain (Cat. No.
ab124812
),
TLR2
(Cat. No.
ab209217
), TLR3 (Cat. No.
ab62566
), and β-actin (Cat. No.
ab115777
) were purchased from
Abcam
(Cambridge, United Kingdom). Animals and
(18)
HCMV-encoded US7 and US8 act as antagonists of innate immunity by distinctively targeting TLR-signaling pathways
Nature Communications
December 1, 2019
Areum Park, Eun A. Ra, Taeyun A. Lee, Hyun jin Choi, Eunhye Lee, Sujin Kang, Jun-Young Seo, Sungwook Lee, Boyoun Park
Tubulin (
G094
,
ABM
Inc.,
1:2000
),
human
TLR3
(
sc-32232
,
Santa Cruz Biotechnology
, Santa Cruz, CA, USA;
ab62566
,
Abcam
, Cambridge, UK,
1:1000
),
human
TLR4
(
sc-293072
,
Santa Cruz Biotechnology
;
312802
,
BioLegend
,
(19)
FOXC1 up-regulates the expression of toll-like receptors in myocardial ischaemia
Journal of cellular and molecular medicine
November 1, 2019
Zhang SP, Yang RH, Shang J, Gao T, Wang R, Peng XD, Miao X, Pan L, Yuan WJ, Lin L,
et al
.
dried milk for 1 hour and further incubated overnight at 4°C with primary antibodies against TLR3 (
ab62566
,
Abcam
),
TLR4
(
NB100‐56566
,
NOVUS
) or
FOXC1
(#
8758
,
Cell Signaling Technology
). After that, the
membrane
(20)
Expression and function of Toll‑like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis
Molecular Medicine Reports
September 2, 2019
Jun Zhang, Rongming Xu, Lei Wu, Jihong Jiang
milk in TBS buffer at 37˚C for 1 h and incubated overnight at 4˚C with antibodies against TLR3 (
1:700
;
ab62566
,
Abcam
), TLR4 (
1:600
;
ab13556
,
Abcam
), TLR5 (
1:800
;
ab62460
,
Abcam
), p65 (
1:1,000
;
ab32536
,
Abcam
),
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Anti-TLR3 antibody from Abcam
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