Anti-Cardiac Troponin T antibody ab45932 from Abcam

Product Specs
ItemAnti-Cardiac Troponin T antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab45932
Antibody TypePrimary
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Sandwich ELISA, Western blot
IsotypeIgG
HostRabbit
ClonalityPolyclonal
Quantity100 UG
Conjugate/TagUnconjugated
Concentration0.4 mg/ml
Gene NameTNNT2
ReactivityHuman
TargetCardiac Troponin T

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Heart and kidney organoids maintain organ-specific function in a microfluidic systemMaterials Today BioDecember 1, 2023Beatrice Gabbin, Viviana Meraviglia, Maricke L. Angenent, Dorien Ward-van Oostwaard, Wendy Sol, Christine L. Mummery, Ton J. Rabelink, Berend J. van Meer, Cathelijne W. van den Berg, Milena Bellinmonoclonal, Sigma-Aldrich Cat. No: A7811 dilution 1:1000) and TNNT2 (rabbit polyclonal Abcam Cat. No: ab45932 dilution 1:1500). The next day, tissues were washed 3 times for 5 min in PBS+/+ and incubated in blocking

(2) In need of age‐appropriate cardiac models: Impact of cell age on extracellular matrix therapy outcomesAging CellNovember 1, 2023S. Gulberk Ozcebe, Pinar Zorlutuna(10% Goat serum) for 1 h at RT. Cells were incubated with the primary antibodies for TNNT2 (Abcam, ab45932), Ki67 (Thermo Fisher, 14–5698‐82), p21 (Abcam, ab54562) (all diluted at 1:150 in goat serum) overnight

(3) Hydrogel‐Sheathed Hipsc‐Derived Heart Microtissue Enables Anchor‐Free Contractile Force MeasurementAdvanced ScienceOctober 17, 2023Yuta Kurashina, Keisuke Fukada, Shun Itai, Shuichi Akizuki, Ryo Sato, Akari Masuda, Hidenori Tani, Jun Fujita, Keiichi Fukuda, Shugo Tohyama, et al.Biosystems, CA, USA). As primary antibodies, α‐actinin (A7811, Sigma), Vimentin (Ab92547, Abcam) and cTnT (Ab45932, Abcam) were used. α‐actinin was employed to observe cytoskeleton and intercellular adhesion, respectively,

(4) Patient-specific iPSC-derived cardiomyocytes reveal aberrant activation of Wnt/β-catenin signaling in SCN5A-related Brugada syndromeStem Cell Research & TherapySeptember 8, 2023Dongsheng Cai, Xiaochen Wang, Yaxun Sun, Hangping Fan, Jingjun Zhou, Zongkuai Yang, Hangyuan Qiu, Jue Wang, Jun Su, Tingyu Gong, et al.μg/ml), OCT4 (Santa Cruz Biotechnology, sc-8628, 1:500), SSEA-4 (Abcam, ab16287, 1:500) TNNT2 (Abcam, ab45932, 1:400), α-actinin (Abcam, ab137346, 1:500), Nav1.5 (Alomone labs, ASC-005, 1:200) and β-catenin (Abcam

(5) Cardiomyocyte proliferation is suppressed by ARID1A-mediated YAP inhibition during cardiac maturationNat CommunAugust 5, 2023Cornelis J. Boogerd, Ilaria Perini, Eirini Kyriakopoulou, Su Ji Han, Phit La, Britt van der Swaan, Jari B. Berkhout, Danielle Versteeg, Jantine Monshouwer-Kloots, Eva van Rooijculture purity was assessed by the percentage of cardiac Troponin T positive cardiomyocytes (Abcam, ab45932; 1:2000). iPS-CM experiments HEK293T Lenti-X cells (TaKaRaBio, Cat# 632180) were transfected with

(6) The mitochondrial calcium uniporter (MCU) activates mitochondrial respiration and enhances mobility by regulating mitochondrial redox stateRedox BiologyAugust 1, 2023Anna Weiser, Aurélie Hermant, Flavien Bermont, Federico Sizzano, Sonia Karaz, Pilar Alvarez-Illera, Jaime Santo-Domingo, Vincenzo Sorrentino, Jerome N. Feige, Umberto De Marchiblocked in 4% BSA (Sigma Aldrich, #A8022) and stained with the primary antibodies anti-troponinT (Abcam, #ab45932, 1/2000) and with appropriate secondary antibodies (Thermo Fisher Scientific, #A-21121). Cell nuclei

(7) SARS-CoV-2 induces cardiomyocyte apoptosis and inflammation but can be ameliorated by ACE inhibitor CaptoprilAntiviral ResearchJuly 1, 2023Xiaohan Huang, Wenxia Fan, Jing Sun, Jiaqing Yang, Yanjun Zhang, Qian Wang, Pingchao Li, Yudi Zhang, Shengnan Zhang, Heying Li, et al.Antibody,Usage,Catalog,Vendor,Dilution anti-a-actinin,IF,A7811,Sigma,1:200 anti-Cardiac Troponin T,IF,ab45932,Abcam,1:200 anti-Troponin T,IF,MS-295-P0,Thermo Fisher Scientific,1:200 anti-ACE2 antibody,IF/WB,ab15348,Abcam,IF

(8) Human induced pluripotent stem cell-derived cardiac myocytes and sympathetic neurons in disease modellingPhilosophical Transactions of the Royal Society B: Biological SciencesJune 19, 2023Ni Li, Michael Edel, Kun Liu, Chris Denning, Jacob Betts, Oliver C. Neely, Dan Li, David J. Patersonwith primary antibodies, anti-α-actinin (Sigma, A7811) and anti-cardiac troponin T (anti-cTnT, Abcam, ab45932), overnight at 4°C. For hiPSC-SN, anti-tyrosine hydroxylase (anti-TH, Sigma, T2928), anti-peripherin

(9) Neonatal and adult cardiac fibroblasts exhibit inherent differences in cardiac regenerative capacityThe Journal of biological chemistryMay 1, 2023Sun H, Pratt RE, Dzau VJ, Hodgkinson CP.overnight at 4 °C with a targeting antibody (Epas1: Novus Biological Cat no NB100-122; Tnnt2: Abcam Cat no ab45932) in antibody buffer. After the overnight incubation, cells were washed three times in an antibody buffer.

(10) Amniotic Fluid Mesenchymal Stromal Cells Derived from Fetuses with Isolated Cardiac Defects Exhibit Decreased Proliferation and Cardiomyogenic PotentialBiology (Basel)April 5, 2023Manali Jain, Neeta Singh, Raunaq Fatima, Aditya Nachanekar, Mandakini Pradhan, Soniya Nityanand, Chandra Prakash Chaturvedi(PE),"330,405",1:100,"BioLegend, San Diego, CA, USA" VEGFR-2 (APC),"359,915",1:100,"BioLegend, San Diego, CA, USA" cTNT,ab45932,1:200,"Abcam, Cambridge, UK" anti-rabbit secondary antibodies (FITC),ab6717,1:200,"Abcam, Cambridge,

(11) Malat1 deficiency prevents neonatal heart regeneration by inducing cardiomyocyte binucleationJCI InsightMarch 8, 2023Galip S. Aslan, Nicolas Jaé, Yosif Manavski, Youssef Fouani, Mariana Shumliakivska, Lisa Kettenhausen, Luisa Kirchhof, Stefan Günther, Ariane Fischer, Guillermo Luxán, et al.software 6.5.1. The following primary and secondary antibodies were used: rabbit anti–troponin T (Abcam, ab45932; 1:1000), rabbit anti–Aurora B (Abcam, ab2254; 1:500), donkey anti-rabbit–Alexa Fluor 555 (Invitrogen,

(12) CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2Circulation researchFebruary 17, 2023Cachorro E, Günscht M, Schubert M, Sadek MS, Siegert J, Dutt F, Bauermeister C, Quickert S, Berning H, Nowakowski F, et al.antibodies were used: CaMKIIδ total (R&D Systems, MAB4176), CaMKII pThr286 (Thermo, MA1-047), cTNT (Abcam, ab45932), PLB (Badrilla, Ltd, A010-14), PLB pSer16 (Badrilla, Ltd, A010-12), PLB pThr17 (Badrilla, Ltd, A010-13),

(13) Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem CellsCell transplantationJanuary 1, 2023Lin CY, Ching YY, Wu SF, Lee YK, Fan HC, Su LY, Tsai SY, Chen YC, Shen CI, Su HL.BioLegend), Actinin (A7811, Sigma-Aldrich), MYH6 (MAB8979, R&D Systems/Biotechne), Troponin T type 2 (TNNT2; ab45932, Abcam, Cambridge, UK), TTN-C (TTN-9, Myomedix, Neckargenmuend, Germany), and Sox17 (ab84990, Abcam).

(14) Single-cell transcriptomic analysis identifies murine heart molecular features at embryonic and neonatal stagesNature communicationsDecember 27, 2022Feng W, Bais A, He H, Rios C, Jiang S, Xu J, Chang C, Kostka D, Li G.Alex488-pHH3 (Abcam ab197502, 1:500 dilution) together with antibodies against lineage genes: cTNT (Abcam, ab45932, 1:400 dilution), Vimentin (Novus Biologicals, NB300-223SS, 1:500 dilution), CD31 (BD, 550274, 1:500

(15) Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarctionNature communicationsDecember 10, 2022Klapproth E, Witt A, Klose P, Wiedemann J, Vavilthota N, Künzel SR, Kämmerer S, Günscht M, Sprott D, Lesche M, et al.BD Biosciences), CD45 (1:100, 555480, BD Biosciences), cTnI (1:100, MAB3150, Millipore), cTnT (1:100, ab45932, Abcam) or Vimentin (1:100, 550513, BD Biosciences) where indicated. Alexa Fluor 488-coupled goat anti-rabbit

(16) A soft and ultrasensitive force sensing diaphragm for probing cardiac organoids instantaneously and wirelesslyNature CommunicationsNovember 25, 2022Quanxia Lyu, Shu Gong, Jarmon G. Lees, Jialiang Yin, Lim Wei Yap, Anne M. Kong, Qianqian Shi, Runfang Fu, Qiang Zhu, Ash Dyer, et al.(Thermo Fisher Scientific) before double stained with cardiac troponin T (2 μg/mL, rabbit polyclonal, ab45932, Abcam, lot#GR3394585-1) and CD31 (2 μg/mL, mouse monoclonal, M0823, clone JC70A, DAKO, lot#00093760)

(17) Induced pluripotent stem cells and cerebral organoids from the critically endangered Sumatran rhinocerosiScienceNovember 18, 2022Zywitza V, Frahm S, Krüger N, Weise A, Göritz F, Hermes R, Holtze S, Colleoni S, Galli C, Drukker M, et al.RRID:AB_2119059 mouse anti-ACTN2,Sigma-Aldrich,Cat#A7811; RRID:AB_476766 rabbit anti-TNNT2,Abcam,Cat#ab45932; RRID:AB_956386 mouse anti-GATA4,Santa Cruz,Cat#sc-25310; RRID:AB_627667 rabbit anti-GATA4,Cell Signaling,Cat#36966;

(18) Systems analysis of de novo mutations in congenital heart diseases identified a protein network in the hypoplastic left heart syndromeCell SystemsNovember 1, 2022Yuejun Jessie Jessie Wang, Xicheng Zhang, Chi Keung Keung Lam, Hongchao Guo, Cheng Wang, Sai Zhang, Joseph C. Wu, Michael Snyder, Jingjing Lidifferentiation efficiency. iPSC-CMs were dissociated and stained with cardiac troponin T antibody (ab45932, Abcam), and goat anti-Rabbit secondary antibody (A32731, Thermo Scientific) using Fixation/Permeabilization

(19) Repeated and On-Demand Intracellular Recordings of Cardiomyocytes Derived from Human-Induced Pluripotent Stem CellsACS SensorsOctober 28, 2022Jihyun Lee, Tobias GÃ¤nswein, Hasan Ulusan, Vishalini Emmenegger, Ardan M. Saguner, Firat Duru, Andreas Hierlemannantibody,target,dilution,supplier,Cat. Hoechst,DNA,1:500,Thermo Fisher Scientific,H3570 anti-cardiac troponin T,cardiac troponin T,1:200,Abcam,AB45932 anti-sarcomeric alpha actinin,sarcomeric alpha actinin,1:200,Abcam,AB9465 "anti-mouse, Alexa Fluor

(20) Metabolic Maturation Increases Susceptibility to Hypoxia-induced Damage in Human iPSC-derived CardiomyocytesStem Cells Translational MedicineOctober 21, 2022Marijn C Peters, Renee G C Maas, Iris van Adrichem, Pieter A M Doevendans, Mark Mercola, Tomo Å ariÄ‡, Jan W Buikema, Alain van Mil, Steven A J Chamuleau, Joost P G Sluijter, et al.A9418). Primary antibodies used were: ACTN1 (Sigma-Aldrich, A7811, 1:200), cardiac troponin T (Abcam, ab45932, 1:100), Ki-67 (Abcam, ab8330, 1:200), pH3 (Cell Signaling Technology, #9701, 1:200), TOMM20 (Abcam,

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Anti-Cardiac Troponin T antibody from Abcam

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Anti-Cardiac Troponin T antibody from Abcam

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