Fig 1: ZAP3 colocalized with cytoplasmic TLS/FUS foci after sorbitol treatment, but m6A-modified RNA fragment impaired this colocalization. (A) RNA Fragment 6 with or without m6A modification was transfected to HAP1 cells prior to 0.4M sorbitol treatment. Representative images of ICC are shown. MERGE images indicate the layered images of TLS/FUS, ZAP3, and DAPI (for nuclei staining). White arrowheads, colocalized TLS/FUS and ZAP3 foci; ?, cells with cytoplasmic ZAP3 foci independent of TLS/FUS. Scale bar = 10 µm. (B,C) Cytoplasmic foci in (A) were quantified. n = 20. unm, unmodified fragment; m6A, m6A-modified fragment.