Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] ab62322 from Abcam

Product Specs
ItemAnti-SHP2 (phospho Y542) antibody [EP508(2)Y]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab62322
Antibody TypePrimary
HostRabbit
CloneEP508(2)Y
IsotypeIgG
ClonalityMonoclonal
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
Quantity10 UL, 40 UL, 100 UL
Conjugate/TagUnconjugated
ModificationsPhospho-Y542
Research AreaImmuno-oncology
Gene NamePTPN11
ReactivityHuman, Mouse
TargetSHP2

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Influences and mechanism of erythropoietin on the cognitive function of vascular dementia ratsAging (Albany NY)November 15, 2023Zhipeng Tang, Xiuqin Li, Nan Yin, Ming Zhao, Qingchuan Hu, Peiyuan Lv(Abcam: ab275686, 1:2000), P22 (Abcam: ab75941, 1:1000), P47 (Abcam: ab308256, 1:1000), p-SHP2 (Abcam: ab62322, 1:1000), p-TrKB (Abcam: ab229908, 1:1000), p-PI3K (Abcam: ab278545, 1:2000), p-ERK1/2 (Abcam: 184699,

(2) Astrocyte interferon-gamma signaling dampens inflammation during chronic central nervous system autoimmunity via PD-L1Journal of NeuroinflammationOctober 12, 2023Brandon C. Smith, Rachel A. Tinkey, Orion D. Brock, Arshiya Mariam, Maria L. Habean, Ranjan Dutta, Jessica L. Williams14-0031-82), -PD-L1 (BioLegend; 124301), -CD45 PerCP-Cy5.5 (BioLegend; 103132), and/or -pSHP2 (Abcam; ab62322) primary antibodies overnight at 4 °C. Secondary antibodies conjugated to Alexa Fluor 488, Alexa Fluor

(3) Myeloid-specific knockout of SHP2 regulates PI3K/PLCγ signaling pathway to protect against early myocardial infarction injuryAging (Albany NY)September 30, 2023Menglin Sha, Hongxing Li, Bingyan Guo, Xiaoyong Gengin the TBST buffer containing 5% skim milk powder, blocked for 2 h. Primary antibodies p-SHP2(Abcam, ab62322, 1:1000), p-PI3K (CellSignaling, 17366, 1:1000), p-MerTK (CellSignaling, 44463,1:1000), p-PLCγ2 (CellSignaling,

(4) Soluble CD4 effectively prevents excessive TLR activation of resident macrophages in the onset of sepsisSignal transduction and targeted therapyJune 19, 2023Zhang SY, Xu QP, Shi LN, Li SW, Wang WH, Wang QQ, Lu LX, Xiao H, Wang JH, Li FY, et al.anti-MHCII (ab180779), anti-MHCII (ab25681), anti-TLR4 (ab22048), anti-SHP2 (ab131541), anti-P-SHP2 (ab62322) mouse IgG isotype control (ab172730), rabbit IgG isotype control (ab37355) from Abcam; STING (66680-1-lg)

(5) Rational combination of SHP2 and mTOR inhibition for the treatment of hepatocellular carcinomaMolecular oncologyJune 1, 2023Mulero-Sánchez A, Ramirez CFA, du Chatinier A, Wang H, Koomen SJI, Song JY, de Groot MHP, Lieftink C, Bosma A, Burylo A, et al.c‐MYC (sc‐764) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibody against p‐SHP2 (ab62322) was obtained from Abcam (Cambridge, UK). Antibodies against GAPDH (#5174), SHP2 (#3752), p‐SHP2 (#3751),

(6) Elaiophylin triggers paraptosis and preferentially kills ovarian cancer drug-resistant cells by inducing MAPK hyperactivationSignal transduction and targeted therapySeptember 12, 2022Li GN, Zhao XJ, Wang Z, Luo MS, Shi SN, Yan DM, Li HY, Liu JH, Yang Y, Tan JH, et al.(Cell Signaling Technology, MA, USA). Primary antibodies against p-IRE1 (S724, ab48187), p-SHP2 (Y542, ab62322), Ki67 (ab16667), MLKL (ab184718), MLKL (S345, ab196436, mouse reactivity), MLKL (S358, ab187091, human

(7) EGFR Inhibition Potentiates FGFR Inhibitor Therapy and Overcomes Resistance in FGFR2 Fusionâ€“Positive CholangiocarcinomaCancer DiscoveryMay 2, 2022Qibiao Wu, Yuanli Zhen, Lei Shi, Phuong Vu, Patricia Greninger, Ramzi Adil, Joshua Merritt, Regina Egan, Meng-Ju Wu, Xunqin Yin, et al.phospho-STAT3 Y705 (9131S, RRID:AB_331586), ERBB3 (12708S, RRID:AB_2721919); from Abcam, phospho-SHP2 Y542 (ab62322, RRID:AB_945452) 1:1000, FRS2 (ab183492) 1:1000, Tubulin (ab6160, RRID:AB_305328) 1:5000; from Sigma

(8) Endothelial Shp2 deficiency controls alternative activation of macrophage preventing radiation-induced lung injury through notch signalingiScienceMarch 18, 2022Liu P, Li Y, Li M, Zhou H, Zhang H, Zhang Y, Xu J, Xu Y, Zhang J, Xia B, et al.3397 Anti-Shp2,Sigma,Cat. # PA5-17956 Anti-Shp2,Santa cruz,Cat. # sc-7384 Anti-Shp2 (phospho Y542),Abcam,Cat. # ab62322 Anti-Jag1,diagbio,Cat. # db8690 Anti-Jag1,Cell SignalingTechnology,Cat. # 70109 Anti-Jag1,R&D,Cat.

(9) SHP2 inhibition improves celastrol-induced growth suppression of colorectal cancerFrontiers in pharmacologyJanuary 1, 2022Zhang L, Hu X, Meng Q, Li Y, Shen H, Fu Y, Zhang F, Chen J, Zhang W, Chang W, et al.Cell Signaling Technology. Antibodies against GAPDH (1:5,000, ab181602) and p-SHP2 (Y542, 1:1,000, ab62322) were purchased from Abcam. Receptor tyrosine kinase arrays Human phospho-RTK arrays (R&D Systems)

(10) Therapeutic Suppression of FAK-AKT Signaling Overcomes Resistance to SHP2 Inhibition in Colorectal CarcinomaFrontiers in PharmacologyNovember 1, 2021Ye Li et al.purchased from Cell Signaling Technologies (CST). GAPDH (1:5000, ab181602) and p-SHP2 (Y542, 1:1000, ab62322) were purchased from Abcam. The primary antibodies above are all from rabbit, so the secondary antibody

(11) Combined administration of SHP2 inhibitor SHP099 and the α7nAChR agonist PNU282987 protect mice against DSS‑induced colitisMolecular medicine reportsSeptember 1, 2020Xiao J, Zhang G, Gao S, Shen J, Feng H, He Z, Xu C.α7nAChR (Sigma-Aldrich; Merck KGaA, cat. no. M220, 1:1,000), phosphorylated (p-)SHP2 (Abcam; cat. no. ab62322, 1:500), SHP2 (Abcam, cat. no. ab131541, 1:500), p-STAT3 (Cell Signaling Technology, Inc.; cat. no.

(12) RUNX2/CBFB modulates the response to MEK inhibitors through activation of receptor tyrosine kinases in KRAS-mutant colorectal cancerTranslational oncologyFebruary 1, 2020Šuštić T, Bosdriesz E, van Wageningen S, Wessels LFA, Bernards R.Santa Cruz Biotechnology. Antibodies against EGFR (ab40815), p-EGFR (Y1068; ab5644), and p-SHP2 (Y542; ab62322) were obtained from Abcam. Antibodies against ERN1 (3294), p-ERK1/2 (9101), RUNX2 (12556), CBFB (12902),

(13) Resistance to allosteric SHP2 inhibition in FGFR-driven cancers through rapid feedback activation of FGFROncotargetJanuary 21, 2020Hengyu Lu, Chen Liu, Hung Huynh, Thi Bich Uyen Le, Matthew J. LaMarche, Morvarid Mohseni, Jeffrey A. Engelman, Peter S. Hammerman, Giordano Caponigro, Huai-Xiang Hao#4060), AKT (CST #9272), FGFR4 (CST #8562), p-EGFR (CST #3777), p-FRS2 (Y436, CST #3861), p-SHP2 (Abcam #ab62322), SHP2 (CST #3397), SPRY4 (R&D System #AF5070), and Tubulin (CST #3873). Phospho-ERK MSD assay Twenty

(14) Antitumor activity of an engineered decoy receptor targeting CLCF1-CNTFR signaling in lung adenocarcinomaNature medicineNovember 1, 2019Kim JW, Marquez CP, Kostyrko K, Koehne AL, Marini K, Simpson DR, Lee AG, Leung SG, Sayles LC, Shrager J, et al.1:1000), P-MEK1/2 (#9121, Cell Signaling, 1:1000), T-MEK1/2 (#9122, Cell Signaling, 1:1000), P-SHP2 (#ab62322, Abcam, 1:1000), T-SHP2 (#3397, Cell Signaling, 1:1000), P-STAT3 (#9145, Cell Signaling, 1:1000), T-STAT3

(15) Inducing and exploiting vulnerabilities for the treatment of liver cancerNatureOctober 1, 2019Wang C, Vegna S, Jin H, Benedict B, Lieftink C, Ramirez C, de Oliveira RL, Morris B, Gadiot J, Wang W, et al.Santa Cruz Biotechnology. Antibodies against CDC7 (ab77668), p-MCM2 (ab109133, ab133243), MCM2 (ab4461), p-SHP2 (ab62322), PCNA (ab2426), and Cleaved caspase-3 (ab2303) were purchased from Abcam. Antibodies against γH2AX

(16) TAS-120 Overcomes Resistance to ATP-Competitive FGFR Inhibitors in Patients with FGFR2 Fusion–Positive Intrahepatic CholangiocarcinomaCancer DiscoveryAugust 1, 2019Lipika Goyal et al.(4060S), AKT (9272S), FGFR1 (9740S); from Abcam (1:5000 dilution): FRS2 (ab183492), phospho-SHP2 Y542 (ab62322); from Sigma (1:20,000 dilution): b-actin (A5316). Quantitative RT-PCR Total RNA from cell lines was

(17) Tyrosyl phosphorylation of KRAS stalls GTPase cycle via alteration of switch I and II conformationNature communicationsJanuary 15, 2019Kano Y, Gebregiworgis T, Marshall CB, Radulovich N, Poon BPK, St-Germain J, Cook JD, Valencia-Sama I, Grant BMM, Herrera SG, et al.(sc-81503, 1:500), and MEK-1 (sc-6250, 1:500) were obtained from Santa Cruz Biotechnology. p-SHP2(Y542) (ab62322, 1:20,000) was obtained from Abcam. Monoclonal antibodies against Pan-Ras (OP40, 1:500), HA (12CA5,

(18) Improved efficiency of in situ protein analysis by proximity ligation using UnFold probesScientific reportsMarch 29, 2018Klaesson A, Grannas K, Ebai T, Heldin J, Koos B, Leino M, Raykova D, Oelrich J, Arngården L, Söderberg O, et al.Cell Signaling Technology) at 1:1000, goat anti Shp2 (PA5–17956, Thermo Fisher) at 1:1000, and rabbit anti pY542 Shp2 (ab62322, Abcam) at 1:5000. Paired combinations of rabbit and mouse, and goat and rabbit primary antibodies were

(19) Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasisNature communicationsDecember 18, 2017Guo W, Liu W, Chen Z, Gu Y, Peng S, Shen L, Shen Y, Wang X, Feng GS, Sun Y, et al.anti-caspase-1 (ab108362, 1 : 1,000 dilution), anti-ANT1 (ab110322, 1 : 1,000 dilution), and anti-p-SHP2 (ab62322, 1 : 500 dilution) were purchased from Abcam (Cambridge, UK). Anti-PYCARD (ASC, sc-271054, 1 : 2,000

(20) Increased Activity of Src Homology 2 Domain Containing Phosphotyrosine Phosphatase 2 (Shp2) Regulates Activity-dependent AMPA Receptor TraffickingThe Journal of biological chemistrySeptember 2, 2016Zhang B, Du YL, Lu W, Yan XY, Yang Q, Yang W, Luo JH.

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1/10/2022Average Anti-SHP2 (phospho Y542) Antibody Raj KumarThe mouse tissues were homogenized, sonicated, and proteins were extracted in RIPA buffer.

Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] from Abcam

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Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] from Abcam

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