Fig 1: Two distinct subsets of macrophages exist in term decidua(A) Heatmap of top marker genes for the decidual HLA-DRlow and HLA-DRhigh subsets, classical and non-classical blood monocytes. Each column is a bin of a fixed number of cells and each row represents a gene. A subset of genes within each cluster is annotated. High and low expression are represented as red and blue, respectively.(B) Violin plots comparing expression of gene markers within decidual macrophage subsets.(C) Surface expression of HLA-DR and CD11c measured by flow cytometry.(D) Brightfield and fluorescence profiles of macrophage subsets captured using imaging flow cytometry.(E) Median Fluorescence Intensities (MFI) of macrophage surface markers on decidual macrophage subsets (n = 7/group).(F) Frequencies of TREM2+ FOLR2+ (n = 5/group) and CD163+ (n = 7/group) within decidual macrophages.(G) Phospho p65 signal in blood monocytes and decidual macrophage subsets (n = 3/group) by flow cytometry. Error bars represent the mean and standard error of the mean (SEM).(H) Comparison of baseline secreted cytokine profiles (median) of sorted decidual macrophage subsets (n = 4/group) and blood monocytes (n = 2). * and # represent significant differences relative to blood monocytes between HLA-DRlow and HLA-DRhigh macrophages, respectively.(I) Responses to overnight lipopolysaccharide (LPS) stimulation of total decidual leukocytes (n = 8/group). Error bars represent the mean and standard error of the mean (SEM).(J) MFI of intracellular cellROX (n = 7/group).(K) Percentage of E. coli pHrodo+ (n = 8/group) in decidual macrophages.(L) Oxygen consumption rate and extracellular acidification rates of decidual macrophages (n = 6/group). ****p < 0.0001, **p < 0.01, *p < 0.05, and #p<0.1.