Fig 1: Silent expression of circCCNB1 promotes the cell cycle of HCC cells by down-expression of GPM6A and up-expression of DYNC1I1 and activation of AKT and ERK signaling pathway. (A) The PPI networks. The PPI network was constructed by GPM6A (green rhombus), ten genes interacting with GPM6A predicted by STRING (pink rectangles), and 145 cell cycle-related genes (yellow Ellipse) derived from PathCards using Cytoscape software. (B) CircCCNB1 silencing inhibited GPM6A expression and up-regulated DYNC1I1 expression by activating the AKT/ERK signaling pathway in HepG2 cells. (C) CircCCNB1 overexpressing promoted GPM6A expression and reduced DYNC1I1 expression by inhibiting the activation of AKT/ERK signaling pathway in HCCLM3 cells. (D) Quantification from B. (E) Quantification from C. Data of the three independent experiments was represented as Mean ± SD. The two-independent t-test was applied for comparing the difference of the gray value of the WB band between two groups (D, E). ns represents not significant, **P<0.01, ****P<0.0001.