Fig 1: Discovery and validation of four-gene signature for TLS identification. (A) Representative H&E image of TLS in CCA. (B) Correlation between TLS presence and genetic mutation. The p values were assessed by chi-square tests. (C) Differential gene expression of TLS-positive versus TLS-negative CCA cases in the TCGA database. (D, E) Representative images of PAX5, TCL1A, TNFRSF13C, and CD79A staining in CCA sections. Scale bars in D, 300 µm. Scale bars in E, 100 µm.