Anti-WDR5 antibody [2C2] ab56919 from Abcam

Product Specs
ItemAnti-WDR5 antibody [2C2]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab56919
Antibody TypePrimary
ClonalityMonoclonal
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
Quantity100 UG
IsotypeIgG2b
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration1 mg/ml
Clone2C2
Research AreaEpigenetics
Gene Namewdr5
ReactivityHuman
TargetWDR5

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) WDR5 is required for DUX4 expression and its pathological effects in FSHD muscular dystrophyNucleic Acids ResearchJune 9, 2023Emanuele Mocciaro, Roberto Giambruno, Stefano Micheloni, Filippo M Cernilogar, Annapaola Andolfo, Cristina Consonni, Maria Pannese, Giulia Ferri, Valeria Runfola, Gunnar Schotta, et al.(Sigma-Aldrich, #RABHRP3), α-Vinculin (Sigma-Aldrich, #V9131), α-BirA (Abcam, #ab232732), α-WDR5 (Abcam, #ab56919). Secondary antibodies conjugated to horseradish peroxidase were used at 1:10 000 dilutions (Jackson Immunoresearch

(2) Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machineryNature CommunicationsDecember 1, 2022Tian Zhou et al.lysate), Anti-KAT3B/p300 antibody (Abcam, ab10485, 2 µg per 107 cell lysate), Anti-WDR5 antibody (Abcam, ab56919, 2 µg per 107 cell lysate), and Anti-DOT1L Antibody (Thermo Fisher, A300-953A, 2 µg per 107 cell lysate).

(3) Histone methyltransferase MLL1 drives renal tubular cell apoptosis by p53-dependent repression of E-cadherin during cisplatin-induced acute kidney injuryCell death & diseaseSeptember 6, 2022Zhang C, Guan Y, Zou J, Yang X, Bayliss G, Zhuang S.RIPA lysis buffer (9806) were purchased from Cell Signaling Technology (MA, USA). Antibodies to WDR5 (ab56919), histone H3 (ab1791), histone H2AX (ab124781), and γ-H2AX (ab81299) were from Abcam (MA, USA). E-cadherin

(4) WDR5-HOTTIP Histone Modifying Complex Regulates Neural Migration and Dendrite Polarity of Pyramidal Neurons via Reelin SignalingMolecular neurobiologyAugust 1, 2022Ka M, Kim HG, Kim WY.as described previously [57,58]. The following primary antibodies were used: Mouse anti-WDR5 (Abcam, AB56919), and rabbit anti-Neu N (Abcam, AB 104225), rabbit anti-c-Myc (Abcam, AB39688), chicken anti-MAP2 (Abcam,

(5) The related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through acetyltransferase-independent mechanismsCell ReportsAugust 24, 2021Veronique Fischer, Damien Plassard, Tao Ye, Bernardo Reina-San-Martin, Matthieu Stierle, Laszlo Tora, Didier DevysRRID:AB_2118292 Rabbit polyclonal anti-HA tag,Abcam,Cat#ab9110; RRID:AB_307019 Mouse monoclonal anti-Wdr5,Abcam,Cat#ab56919; RRID:AB_946146 Mouse monoclonal anti-Tbp,In-house,"3TF1–3G3; Brou et al., 1993" Rabbit polyclonal

(6) HOTTIP lncRNA Promotes Hematopoietic Stem Cell Self-Renewal Leading to AML-like Disease in MiceCancer cellDecember 9, 2019Luo H, Zhu G, Xu J, Lai Q, Yan B, Guo Y, Fung TK, Zeisig BB, Cui Y, Zha J, et al.polyclonal",Novus Biologicals,"Cat#NB600–248, RRID:AB_2145479" "Anti-WDR5 antibody, mouse monoclonal",Abcam,"Cat#ab56919, RRID:AB_946146" "Anti-DOT1L antibody, rabbit polyclonal",Novus Biologicals,"Cat# NB100–40845, RRID:AB_789636" "Anti-p-STAT5A,

(7) Mll‐COMPASS complexes mediate H3K4me3 enrichment and transcription of the osteoblast master gene Runx2/p57 in osteoblastsJournal of Cellular PhysiologyMay 1, 2019Adriana Rojas et al.RNA-PolII N-20 (sc-899, Santa Cruz Biotechnology), Runx2 S-19 (sc-12488, Santa Cruz Biotechnology), Wdr5 (ab56919, Abcam), JARID1B/KDM4B (ab50958, Abcam), Ezh2 (39901, Active Motif, Carlsbad, CA, USA), Utx/Kdm6A (ab91231,

(8) Divergent lncRNA GATA3-AS1 Regulates GATA3 Transcription in T-Helper 2 CellsFrontiers in ImmunologyOctober 29, 2018Hunter R. Gibbons, Guzel Shaginurova, Laura C. Kim, Nathaniel Chapman, Charles F. Spurlock, Thomas M. Aunefollowed by incubation with an isotype IgG control antibody (sc2025, SantaCruz Biotech), anti-WDR5 (ab56919, Abcam), or anti-p300 (ab14984, Abcam) overnight at 4°C. Protein A/G beads (sc2003, SantaCruz Biotech)

(9) Downregulation of long noncoding RNA FENDRR predicts poor prognosis in renal cell carcinomaOncology LettersOctober 26, 2018Wang He, Guangzheng Zhong, Pei Wang, Chun Jiang, Ning Jiang, Jian Huang1:500 dilution; cat. no. ab12073, ChIP grade) and WD repeat domain 5 (WDR5; 1:500 dilution; cat. no. ab56919; ChIP grade; all Abcam, Cambridge, UK), and incubated with agitation overnight at 4°C. The retrieved

(10) H3K4me2 and WDR5 enriched chromatin interacting long non-coding RNAs maintain transcriptionally competent chromatin at divergent transcriptional unitsNucleic Acids ResearchOctober 12, 2018Santhilal Subhash, Kankadeb Mishra, Vijay Suresh Akhade, Meena Kanduri, Tanmoy Mondal, Chandrasekhar Kandurias per the published protocol (32) using antibodies anti-H3K4me2 (Abcam: ab7766), anti-WDR5 (Abcam: ab56919) and anti-IgG (Millipore; 12-370). In brief, BT-549 cells were treated with 5 μg/μl of Actinomycin D

(11) Three classes of response elements for human PRC2 and MLL1/2–Trithorax complexesNucleic Acids ResearchSeptember 28, 2018Junqing Du, Brian Kirk, Jia Zeng, Jianpeng Ma, Qinghua Wang(Catalog # SC-67105); anti-EED from Millipore (Catalog # 05-1320); anti-WDR5 from Abcam (Catalog # ab56919); anti-MLL1 from Santa Cruz (Catalog # SC-20153); anti-Tubulin from Sigma (Catalog # T8328), anti-TATA

(12) Dppa2/4 Facilitate Epigenetic Remodeling during Reprogramming to PluripotencyCell stem cellSeptember 6, 2018Hernandez C, Wang Z, Ramazanov B, Tang Y, Mehta S, Dambrot C, Lee YW, Tessema K, Kumar I, Astudillo M, et al.2191856 Rabbit polyclonal anti-Kdm2a,Abcam,Cat# ab31739;RRID:AB 775817 Mouse monoclonal anti-Wdr5,Abcam,Cat#ab56919;RRID:AB 946146 Rabbit polyclonal anti-Rbbp5,Abcam,Cat# ab154755;RRID:AB 2728762 Rabbit polyclonal

(13) Long non‐coding RNA CASC 15 regulates gastric cancer cell proliferation, migration and epithelial mesenchymal transition by targeting CDKN 1A and ZEB 1Molecular OncologyJune 1, 2018Qiong Wu, Shihao Xiang, Jiali Ma, Pingping Hui, Ting Wang, Wenying Meng, Min Shi, Yugang Wang(Millipore) on the basis of the manufacturer's guidance. Antibodies for EZH2 (cat#: ab186006) and WDR5 (cat#: ab56919) were acquired from Abcam. RT‐PCR was used to detect the co‐precipitated RNA. 2.11 Dual luciferase reporter assay

(14) TWIST1-WDR5-Hottip Regulates Hoxa9 Chromatin to Facilitate Prostate Cancer MetastasisCancer researchJune 15, 2017Malek R, Gajula RP, Williams RD, Nghiem B, Simons BW, Nugent K, Wang H, Taparra K, Lemtiri-Chlieh G, Yoon AR, et al.(ab92547; Abcam), ZO-1 (5406; Cell Signaling Technology), β-actin (A5316; Santa Cruz Biotechnology), WDR5 (ab56919; Abcam), H3K4me3 (ab8580; Abcam), H3K27me3 (ab6002; Abcam), and horseradish peroxidase-conjugated secondary

(15) INO80 is Required for Osteogenic Differentiation of Human Mesenchymal Stem CellsScientific ReportsDecember 1, 2016Chenchen Zhou, Jing Zou, Shujuan Zou, Xiaobing Lilegends. Antibodies information is listed as below: INO80 (1:1000, ab22238, Abcam, China), Wdr5 (1:1,000, ab56919, Abcam, China). All of the IP experiments were repeated at least three times. Xenograft model for MSC-mediated

(16) The long noncoding RNA Chaer defines an epigenetic checkpoint in cardiac hypertrophyNature MedicineOctober 1, 2016Zhihua Wang et al.

(17) Polycomb PRC2 complex mediates epigenetic silencing of a critical osteogenic master regulator in the hippocampusBiochimica et Biophysica Acta (BBA) - Gene Regulatory MechanismsAugust 1, 2016Rodrigo Aguilar, Fernando J. Bustos, Mauricio Saez, Adriana Rojas, Miguel L. Allende, Andre J. van Wijnen, Brigitte van Zundert, Martin Montecinomonoclonal anti JBP1/ PRMT5 (611539, BD Transduction Laboratories, CA, USA), monoclonal anti Wdr5 (ab56919, Abcam), monoclonal anti Suv39H1 (ab12405, Abcam), polyclonal anti UTX (ab36938, Abcam), polyclonal

(18) An epigenetic mechanism mediates developmental nicotine effects on neuronal structure and behaviorNature NeuroscienceJuly 1, 2016Yonwoo Jung et al.(Cell Signaling, 5030) were validated using cells overexpressing Ash2l and Mef2c. Anti-Wdr5 antibody (Abcam, ab56919) and anti-Rbbp5 antibody (Abcam, ab52084) have been validated in published studies (supplementary methods

(19) Genome-Wide Studies Reveal that H3K4me3 Modification in Bivalent Genes Is Dynamically Regulated during the Pluripotent Cell Cycle and Stabilized upon DifferentiationMolecular and Cellular BiologyFebruary 15, 2016Rodrigo A. Grandy et al.anti-KDM5A (Abcam; catalog no. ab70892, lot no. GR70517-14/GR70517-24), anti-WDR5 (Abcam; catalog no. ab56919, lot no. GR186228), anti-MLL1-473 (kindly donated by Eli Canaani, Weizmann Institute, Israel), anti-MLL2

(20) Epigenetic Control of the Bone-master Runx2 Gene during Osteoblast-lineage Commitment by the Histone Demethylase JARID1B/KDM5BThe Journal of biological chemistryNovember 20, 2015Adriana Rojas, Rodrigo Aguilar, Berta Henriquez, Jane B Lian, Janet L Stein, Gary S Stein, Andre J van Wijnen, Brigitte van Zundert, Miguel L Allende, Martin Montecino

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Anti-WDR5 antibody [2C2] from Abcam

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Anti-WDR5 antibody [2C2] from Abcam

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