Anti-SOX9 (phospho S181) antibody ab59252 from Abcam

Product Specs
ItemAnti-SOX9 (phospho S181) antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab59252
Antibody TypePrimary
Quantity100 UG
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
HostRabbit
ClonalityPolyclonal
IsotypeIgG
Conjugate/TagUnconjugated
FormatAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
Concentration1 mg/ml
ModificationsPhospho-S181
Gene NameSox9
ReactivityHuman
TargetSOX9

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Platelet-Derived Biomaterials Exert Chondroprotective and Chondroregenerative Effects on Diabetes Mellitus-Induced Intervertebral Disc DegenerationLife (Basel, Switzerland)October 7, 2021Lo WC, Chang CC, Chan CH, Singh AK, Deng YH, Lin CY, Tsao W, Chien ST, Lin CH, Deng WP.h at room temperature. The membranes were further incubated with primary antibodies including Sox9 (ab59252, 1:500, Abcam, Cambridge, UK), type 2 collagen (Col II) (ab34712, 1:1000, Abcam), AGN (MABT83, 1:500,

(2) Platelet-Derived Biomaterials Inhibit Nicotine-Induced Intervertebral Disc Degeneration Through Regulating IGF-1/AKT/IRS-1 Signaling AxisCell transplantationJanuary 1, 2021Lo WC, Chiou CS, Tsai FC, Chan CH, Mao S, Deng YH, Wu CY, Peng BY, Deng WP.at room temperature to avoid non-specific binding. The membranes were further incubated with Sox9 (ab59252, 1:500, Abcam, Cambridge, UK), Col II (ab34712, 1:1000, Abcam), AGN (MABT83, 1:500, Millipore, Billerica,

(3) Agonism of GPR120 prevented IL-1β-induced reduction of extracellular matrix through SOX-9AgingJune 24, 2020Xu Z, Ke T, Zhang Y, Fu C, He W.collagen (1: 500, #MAB8887, Chemicon, USA); aggrecan (1: 500, #ab3778, Abcam, USA); SOX-9 (1:1000, #ab59252, Abcam, USA); β-actin (1: 10000, #ab8227, Abcam, USA); NF-κB p65 (1:1000, #ab16502, Abcam, USA); Lamin

(4) Amelioration of Nicotine-Induced Osteoarthritis by Platelet-Derived Biomaterials Through Modulating IGF-1/AKT/IRS-1 Signaling AxisCell transplantationJanuary 1, 2020Lo WC, Dubey NK, Tsai FC, Lu JH, Peng BY, Chiang PC, Singh AK, Wu CY, Cheng HC, Deng WP.blocked with 4% bovine serum albumin (BSA) blocking buffer. The membrane was then reacted with Sox9 (ab59252, 1:500, Abcam, Cambridge, UK), Col II (ab34712, 1:1000, Abcam), IGF-1 R (9750 S, 1:500, Cell Signaling,

(5) Dynamic Expression Profiles of Sox9 in Embryonic, Post Natal, and Adult Heart Valve Cell PopulationsAnatomical record (Hoboken, N.J. : 2007)January 1, 2019Gallina D, Lincoln J.ab26414, 1:50), Sox9 (173–185aa) (Rabbit, Abcam, ab3697, 1:50), phospho(181) Sox9 (pSox9) (Rabbit, Abcam, ab59252, 1:50), CD31 (Rabbit, Abcam, ab28364, 1:20, and Goat, Santa Cruz, sc-1506, 1:100), Col2 (Rabbit, Abcam,

(6) Highly Proliferative α-Cell-Related Islet Endocrine Cells in Human PancreataDiabetesApril 1, 2018Lam CJ, Cox AR, Jacobson DR, Rankin MM, Kushner JA.(18-0078; Invitrogen), synaptotagmin 1A (ab133856; Abcam), Sox9 (AB5535; Millipore), Sox9 (pS181) (ab59252; Abcam), and synaptophysin (18-0130, Thermo Fisher Scientiﬁc, and AB6245, Abcam); 1:250, glucagon (ab8055

(7) Expression of Pluripotency Genes in Chondrocyte-Like Cells Differentiated from Human Induced Pluripotent Stem CellsInternational Journal of Molecular SciencesFebruary 12, 2018Ewelina Stelcer, Katarzyna Kulcenty, Marcin Rucinski, Karol Jopek, Tomasz Trzeciak, Magdalena Richter, Joanna P. Wroblewska, Wiktoria M. Suchorska

(8) Gene expression profile in human induced pluripotent stem cells: Chondrogenic differentiation in vitro, part AMolecular Medicine ReportsMay 1, 2017Wiktoria Maria Suchorska, Ewelina Augustyniak, Magdalena Richter, Tomasz Trzeciakab134568), agreccan (AGC1; 1:85; cat. no. ab3778), SOX6 (1:50; cat. no. ab30455), SOX9 (1:50; cat. no. ab59252); all from Abcam, Cambridge, UK), Nanog (1:50; cat. no. MABD24) and octamer-binding transcription factor

(9) Anti-IL-20 monoclonal antibody inhibited inflammation and protected against cartilage destruction in murine models of osteoarthritisPLOS ONEApril 20, 2017Yu-Hsiang Hsu, Ya-Yu Yang, Man-Hsiang Huwang, Yun-Han Weng, I-Ming Jou, Po-Tin Wu, Tain-Yu Lin, Li-Wha Wu, Ming-Shi Changprotein was loaded in each experiment. Immunoblotting was done using antibody against phospho-Sox9 (ab59252; Abcam Plc, Cambridge, UK). β-actin (Cell Signaling Technology) was used as loading controls. Quantification

(10) Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs)Stem cell reviewsApril 1, 2017Suchorska WM, Augustyniak E, Richter M, Trzeciak T.type IX collagen (1:100) (ab134568); aggrecan (1:85) (ab3778); SOX6 (1:50) (ab30455); SOX9 (1:50) (ab59252); E- cadherin (ab152102) as well as Nanog (1:50) (MABD24) and OCT3/4 (1:50) (MABD76) (both from Merck Millipore

(11) SOX9-regulated cell plasticity in colorectal metastasis is attenuated by rapamycinScientific ReportsOctober 1, 2016Estefania Carrasco-Garcia et al.

(12) Signaling Cascades Governing Cdc42-Mediated Chondrogenic Differentiation and Mensenchymal CondensationGeneticsMarch 1, 2016Wang JR, Wang CJ, Xu CY, Wu XK, Hong D, Shi W, Gong Y, Chen HX, Long F, Wu XM.Antibodies against AP (ab95462), BSP (ab33022), Cdc42 (ab64533), OC (ab13420), Osx (ab22552), p-Sox9 (ab59252), Sox9 (ab26414), and Runx2 (ab76956) were purchased from Abcam (Cambridge, UK). The IRDye 680- and

(13) Contribution of the Interleukin-6/STAT-3 Signaling Pathway to Chondrogenic Differentiation of Human Mesenchymal Stem CellsArthritis & rheumatology (Hoboken, N.J.)May 1, 2015Masahiro Kondo, Kunihiro Yamaoka, Kei Sakata, Koshiro Sonomoto, Lin Lin, Kazuhisa Nakano, Yoshiya Tanaka

(14) Decreased adipogenesis and adipose tissue in mice with inactivated protein phosphatase 5The Biochemical journalFebruary 15, 2015Jacob W, Rosenzweig D, Vázquez-Martin C, Duce SL, Cohen PT.

(15) Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesisJournal of Tissue EngineeringJanuary 16, 2015Sanjukta Guha Guha Thakurta, Gaurav Budhiraja, Anuradha Subramanian376969), COL10A1 (ABCAM; ab182563), SOX-9 (Millipore-ab5535), phospho S181-SOX-9 or p-SOX-9 (ABCAM; ab59252), and Aggrecan (ABCAM; ab3773). β-Actin was used as the respective loading control. After washing the

(16) The role of Sox9 in mouse mammary gland development and maintenance of mammary stem and luminal progenitor cellsBMC developmental biologyDecember 20, 2014Malhotra GK, Zhao X, Edwards E, Kopp JL, Naramura M, Sander M, Band H, Band V.(Millipore Cat: AB5535; LSBio Cat: LS-B5761/29528; Abnova, Cat: PAB12736; AbCam Cat: ab3697, ab71762, Ab59252) but were unable to detect specific Sox9 staining in luminal cells. The antibodies tested showed either

(17) IL-17 Inhibits Chondrogenic Differentiation of Human Mesenchymal Stem CellsPLoS ONENovember 15, 2013Masahiro Kondo, Kunihiro Yamaoka, Koshiro Sonomoto, Shunsuke Fukuyo, Koichi Oshita, Yosuke Okada, Yoshiya TanakaBeverly, MA), phospho-SMAD2 (138D4, Cell Signaling Technology), SOX9 (ab26414, Abcam) or phospho-SOX9 (ab59252, Abcam) followed by appropriate secondary antibodies (GE Healthcare, Little Chalfont, UK). β-actin (A-1978,

(18) Phosphorylation of Sox9 is required for neural crest delamination and is regulated downstream of BMP and canonical Wnt signalingProceedings of the National Academy of Sciences of the United States of AmericaFebruary 19, 2013Jessica A J Liu, Ming-Hoi Wu, Carol H Yan, Bolton K H Chau, Henry So, Alvis Ng, Alan Chan, Kathryn S E Cheah, James Briscoe, Martin Cheung

(19) Fibroblast Growth Factor-2 Primes Human Mesenchymal Stem Cells for Enhanced ChondrogenesisPLoS ONEJuly 27, 2011Andrew M. Handorf, Wan-Ju Li

(20) Cyclic GMP-dependent protein kinase II inhibits cell proliferation, Sox9 expression and Akt phosphorylation in human glioma cell linesOncogeneSeptember 3, 2009Swartling FJ, Ferletta M, Kastemar M, Weiss WA, Westermark B.

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Anti-SOX9 (phospho S181) antibody from Abcam

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Anti-SOX9 (phospho S181) antibody from Abcam

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