Fig 1: Relative levels of CCNA2 and CCNB1 following wash-out from nocodazole arrest.Mitotically arrested HeLa cells were washed out from nocodazole for 25 minutes prior to CDK inhibition with flavopiridol. Samples were harvested as indicated and analysed by Western blot, with 8 µg of total lysate loaded per lane. Film exposure times are indicated next to the corresponding label. Antibodies and dilutions used are as follows: PRC1-pT481 1:4000 (AbCam; EP1514Y/ab62366), Cyclin A2 1:2000 (AbCam; ab32498), Cyclin B1 1:5000 (Millipore; 05-373), Tubulin 1:10,000 (Sigma; T6199).