Fig 2: Effects of Sirt7 knockdown on cell proliferation and colony formation ability. (A) MTS assays on U87 and U251 cells following transfection with si-Sirt7 or negative control siRNA. (B) Downregulation of Sirt7 inhibited glioma cell line colony formation ability in U87 and U251 cell lines. **P<0.01, *P<0.05. Sirt7, sirtuin 7; siRNA, small interfering RNA; si-Sirt7, siRNA targeting Sirt7; siC, control siRNA.

Fig 3: Expression of SIRT7 in the kidney. (A) SIRT7 expression in mouse kidney, liver, and heart was evaluated. GAPDH and ß-actin were used as loading controls. SIRT7 protein expression was deleted in Sirt7 KO mouse kidney. (B) SIRT7 expression in the kidney of Sirt7 FRT/floxed mutant mouse was evaluated by ß-galactosidase staining. (C) Representative photomicrographs (×400) of immunohistochemical staining for SIRT7 in kidney sections. Scale bar: 50 µm. (D) Representative photomicrographs (×400) of double immunostaining (lectin and SIRT7). PHA-E was used as a marker of proximal tubules and DBA was used as a marker of collecting tubules. The arrows indicate SIRT7-expressing nuclei. Scale bars: 50 µm.

Fig 4: Proposed model of how SIRT7 deficiency protects against Aβ42-induced neuronal cell death.

Fig 5: Colocalization of Dicer and SIRT7 in the cytoplasm. (A) Subcellular localization of Dicer, SIRT7, H3K18Ac, histone H3 and lamin A/C, revealed by biochemical fractionation. Upper panel, the schematic diagram of biochemical fractionation assay; bottom panel, the representative western blot images. S2, S3 and S4 represent the cytoplasmic, the nucleoplasmic and the chromatin-associated fractions, respectively. (B and C) Colocalization of Dicer and SIRT7 in the cytoplasm revealed by immunofluorescence using anti-Dicer and two different anti-SIRT7 antibodies. (B) NB110–81762; (C) 5360.