Fig 1: The integrin subunits ITGAV and ITGB5 can be used to enrich for human pancreatic endocrine cells. (a) Unsupervised clustering UMAP projection plots for four independent human donors, with cell types as indicated (refer to Supplementary Fig. 1 for parameters used to assign cell cluster identity). (b) Flow cytometry plot showing sort gates for human islets stained with antibodies directed against integrins av and ß5. Populations representing avhiß5hi (shaded green), avloß5lo (shaded orange), av+ß5neg (shaded red) were subjected to RNAseq analysis. (c) Heatmap showing expression of the top 20 highly expressed genes in the sorted avloß5lo population mapped against cell clusters from the scRNAseq analysis shown in (a). (d) Top 20 most highly expressed genes in each of the avhiß5hi, avloß5lo and av+ß5neg sorted populations. (e) Transcript quantification (counts per million, CPM) of INSULIN expression in each of the avhiß5hi, avloß5lo and av+ß5neg sorted populations.